Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 3893118..3893812 Replicon chromosome
Accession NZ_CP062811
Organism Escherichia coli strain Res13-Sevr-PER07-33

Toxin (Protein)


Gene name yafO Uniprot ID Q0T7Q5
Locus tag IMQ59_RS18600 Protein ID WP_001263491.1
Coordinates 3893118..3893516 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag IMQ59_RS18605 Protein ID WP_000554758.1
Coordinates 3893519..3893812 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
- 3888706..3888786 - 81 NuclAT_11 - -
- 3888706..3888786 - 81 NuclAT_11 - -
- 3888706..3888786 - 81 NuclAT_11 - -
- 3888706..3888786 - 81 NuclAT_11 - -
IMQ59_RS18575 3889382..3889840 - 459 WP_001291990.1 xanthine phosphoribosyltransferase -
IMQ59_RS18580 3890101..3891558 + 1458 WP_001293009.1 cytosol nonspecific dipeptidase -
IMQ59_RS18585 3891615..3892136 - 522 Protein_3642 peptide chain release factor H -
IMQ59_RS18590 3892135..3892338 - 204 Protein_3643 RNA ligase RtcB family protein -
IMQ59_RS18595 3892656..3893108 - 453 WP_071591616.1 GNAT family N-acetyltransferase -
IMQ59_RS18600 3893118..3893516 - 399 WP_001263491.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
IMQ59_RS18605 3893519..3893812 - 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
IMQ59_RS18610 3893864..3894919 - 1056 WP_001226155.1 DNA polymerase IV -
IMQ59_RS18615 3894990..3895775 - 786 WP_000207555.1 putative lateral flagellar export/assembly protein LafU -
IMQ59_RS18620 3895747..3897459 + 1713 Protein_3649 flagellar biosynthesis protein FlhA -
IMQ59_RS18625 3897675..3898172 - 498 WP_001624615.1 REP-associated tyrosine transposase RayT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15457.86 Da        Isoelectric Point: 8.0949

>T176651 WP_001263491.1 NZ_CP062811:c3893516-3893118 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T176651 NZ_CP082860:3713411-3713629 [Enterobacter ludwigii]
ATGTCTGAAAAACCACTCACAAAAATCGATTATTTGATGCGTTTGCGACGCTGTCAGTCAATAGACACGCTTGAACGTGT
AATTGAAAAGAATAAATACGAGCTTTCTGATAATGAACTGGCGGTATTTTATTCAGCGGCTGACCACCGTCTTGCAGAAC
TGACTATGAATAAGCTGTATGACAAAATCCCAACATCCGTGTGGAGATTTGTCCGTTAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT176651 WP_000554758.1 NZ_CP062811:c3893812-3893519 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT176651 NZ_CP082860:3713009-3713383 [Enterobacter ludwigii]
ATGGACGAGTACTCGCCAAAAAGGCATGATATCGCGCAGTTGAAATTTCTCTGCGAATCCTTGTACCATGACTGCCTTGC
CAATCTTGATGAAAGTAACCATGGCTGGGTAAACGACCCAACGTCTGCCATCAATTTACAGTTAAACGAGTTGATCGAGC
ATATTGCAACCTTCGCACTTAATTATAAAATTAAGTACAATGAAGATAATAAGCTGATTGAGCAAATTGATGAATACCTG
GATGACACCTTTATGTTGTTCAGCAGCTACGGCATTAATGCGCAAGATTTGCAGAAATGGCGTAAATCGGGCAACCGACT
ATTTCGTTGCTTCATCAACGTGAGCAGAGCTAACCCCGTTAGTCTTTCCTGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TPK7

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References