Detailed information of TA system TA176624

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 118392..118997
• Replicon: chromosome
• Accession: NZ_CP062804
• Organism: Cupriavidus basilensis strain DSM 11853

Toxin (Protein)

• Gene name: graT
• Uniprot ID: -
• Locus tag: F7R26_RS21480
• Protein ID: WP_150986485.1
• Coordinates: 118392..118670 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: F7R26_RS21485
• Protein ID: WP_150986486.1
• Coordinates: 118683..118997 (+)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F7R26_RS21460	114112..114837	+	726	WP_150986481.1	FadR family transcriptional regulator	-
F7R26_RS21465	114900..115937	-	1038	WP_150986482.1	hypothetical protein	-
F7R26_RS21470	116119..117501	+	1383	WP_150986483.1	ethanolamine ammonia-lyase subunit EutB	-
F7R26_RS21475	117549..118337	+	789	WP_150986484.1	ethanolamine ammonia-lyase subunit EutC	-
F7R26_RS21480	118392..118670	+	279	WP_150986485.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
F7R26_RS21485	118683..118997	+	315	WP_150986486.1	HigA family addiction module antidote protein	Antitoxin
F7R26_RS21490	119037..120453	-	1417	Protein_104	FAD-binding oxidoreductase	-
F7R26_RS21495	120558..121589	-	1032	WP_150986487.1	alpha/beta fold hydrolase	-
F7R26_RS21500	121646..123244	-	1599	WP_183034791.1	PLP-dependent aminotransferase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (23-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (21-68)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10425.97 Da
• Isoelectric Point: 9.3525

>T176624 WP_150986485.1 NZ_CP062804:118392-118670 [Cupriavidus basilensis]
MIQSFKCEDTAALFSGRRVPRFANIRAAAERKLEMLAVAASLDFLRSPPGNRLEVLGGDRKGQHSIRINDRWRVCFVWTP
DGPQAVEIVDYH

Nucleotide

• Length: 279 bp

>T176624 NZ_CP082847:791569-791790 [Legionella pneumophila]
ATGATTTTTATTGAAACTCCAATTTTTACAGAAGACGTGAAGGAATTATTATCTGATGAGGAGTATGCTGAGTTTCAAGA
GTATCTTGCTGATAATCCTTTAGCTGGAGACGTAATTCAGCAAACTGGCAAACTGGCAAACTGGCAAACTGCGAAAAGTT
CGATGGTCATTTCAAGGCAAATGGGAAGCGAGGCGGTGTTAGAATTATTTATTATTATGTAA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 11589.26 Da
• Isoelectric Point: 7.4036

>AT176624 WP_150986486.1 NZ_CP062804:118683-118997 [Cupriavidus basilensis]
MARFHNGMRPVHPGEILREEFLVPLGMSANALAVALHVTAARINDIVRERRGITPDTAMRLARYFGGDAESWLNLQQSYD
LKVAQREHGKEIAAQIAPRDLEAA

Nucleotide

• Length: 315 bp

>AT176624 NZ_CP082847:791900-792190 [Legionella pneumophila]
ATGGATAAAAAATTATTTGAACGATTATACGAAAGTATGACTCAGATGAACGAAATTATTGATGGTGAGCGTGTTCCTTC
ACGTGAGTTCGTAGTGGATGCTGTTCATGTAAAAGAAATTAGAAAAAAAACAGGCCTAACCCAAGAAAAATTTTGTCATT
TAATTGATGTAAGTATTGGAACATTAAGAAATTGGGAGCAGGGGAGAAGAGAGCCAACAGGTCCTGCAAAAGCTCTTCTT
CGCGCAATAAATAAAGATCCTGAACATGTATTAGCAGCATTAGCTGGTTGA