Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 3817935..3818532 Replicon chromosome
Accession NZ_CP062792
Organism Klebsiella pneumoniae strain 33Kpn12

Toxin (Protein)


Gene name higB Uniprot ID R4YIC5
Locus tag IFY65_RS18920 Protein ID WP_004142563.1
Coordinates 3818215..3818532 (-) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID R4YH91
Locus tag IFY65_RS18915 Protein ID WP_004142561.1
Coordinates 3817935..3818222 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IFY65_RS18885 3814015..3814263 + 249 WP_020325243.1 DUF1158 domain-containing protein -
IFY65_RS18890 3814281..3814622 - 342 WP_002893035.1 RamA family antibiotic efflux transcriptional regulator -
IFY65_RS18895 3814653..3815768 - 1116 WP_020325248.1 MBL fold metallo-hydrolase -
IFY65_RS18900 3815948..3816529 + 582 WP_020325240.1 TetR/AcrR family transcriptional regulator -
IFY65_RS18905 3816529..3816897 + 369 WP_020325252.1 MmcQ/YjbR family DNA-binding protein -
IFY65_RS18910 3817017..3817670 + 654 WP_004178896.1 oxygen-insensitive NAD(P)H nitroreductase -
IFY65_RS18915 3817935..3818222 - 288 WP_004142561.1 helix-turn-helix transcriptional regulator Antitoxin
IFY65_RS18920 3818215..3818532 - 318 WP_004142563.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
IFY65_RS18925 3818717..3819760 - 1044 WP_020802450.1 DUF2157 domain-containing protein -
IFY65_RS18930 3820430..3821296 - 867 WP_004151823.1 helix-turn-helix transcriptional regulator -
IFY65_RS18935 3821405..3822832 + 1428 WP_009308097.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-88)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12110.39 Da        Isoelectric Point: 11.2767

>T176617 WP_004142563.1 NZ_CP062792:c3818532-3818215 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPPIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Download         Length: 318 bp

>T176617 NZ_CP082844:7080901-7081293 [Nocardia asteroides]
GTGAAGGCCGAACGCGCACTCGCGGACACCTCGATATTCATAGGTCTCGAGGCAGAGCGTTTCGACCCGGCACGATTCGA
CGGATACGAGTGGGGTGTTTCGGTCATCACCCTCGGCGAACTACGTCTCGGTGTGCTGCGGGCCGCGAGCCCGGACGTAT
CGGCCCGCAGGCTGGCGACCTACCAGCTCGCGAAACAGTTCCAGCCACTGGAACTGGACGAGGCCGTCTCGGAGCACTGG
GCGCTGCTGGTCGCGCGTCTGCGAGCCGCCGGGCGCAAGGTGCCGATCAACGACAGTTGGATCGCGGCGACCGCGCTGGC
ACACGATATTCCGATTGTGACCCAGGATGGCGATTACGACGCGATGCCCGACCTGAAGGTGCTGAAGTTGTAG

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10771.46 Da        Isoelectric Point: 10.2212

>AT176617 WP_004142561.1 NZ_CP062792:c3818222-3817935 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Download         Length: 288 bp

>AT176617 NZ_CP082844:7080650-7080904 [Nocardia asteroides]
ATGACTGCGCTACCCGCTCGCGACTTGCGAAATCACACCGCCGAGGTATTGCGCCGCGTCGAGGCAGGCGAGGAGATCGA
GATCCTCAAGGACAATCGGCCCGTCGCGAAGATCATCCCGTTACCTCGGCGTCGACAGTGGGTGCCCGCCCCGGAAATCC
TTCGCGAACTGGTTCGCCTCGGCCCGGACACCACAGGCCTGCACCAGGATCTCCGTGAGACGCTGACCGATACGACGGAC
GACTTGCCGTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M5MXH8

Antitoxin

Source ID Structure
AlphaFold DB A0A0S3DIQ1

References