Detailed information of TA system TA176589

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 4592843..4593537
• Replicon: chromosome
• Accession: NZ_CP062782
• Organism: Escherichia coli O157:H7 strain Z869

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: C3TNX7
• Locus tag: ILQ35_RS23410
• Protein ID: WP_001263495.1
• Coordinates: 4592843..4593241 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: ILQ35_RS23415
• Protein ID: WP_000554757.1
• Coordinates: 4593244..4593537 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
-	4588430..4588510	-	81	NuclAT_12	-	-
-	4588430..4588510	-	81	NuclAT_12	-	-
-	4588430..4588510	-	81	NuclAT_12	-	-
-	4588430..4588510	-	81	NuclAT_12	-	-
ILQ35_RS23385	4589106..4589564	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
ILQ35_RS23390	4589825..4591282	+	1458	WP_001293009.1	cytosol nonspecific dipeptidase	-
ILQ35_RS23395	4591339..4591860	-	522	Protein_4580	peptide chain release factor H	-
ILQ35_RS23400	4591859..4592062	-	204	Protein_4581	RNA ligase RtcB family protein	-
ILQ35_RS23405	4592381..4592833	-	453	WP_001059871.1	GNAT family N-acetyltransferase	-
ILQ35_RS23410	4592843..4593241	-	399	WP_001263495.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
ILQ35_RS23415	4593244..4593537	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
ILQ35_RS23420	4593589..4594644	-	1056	WP_001226186.1	DNA polymerase IV	-
ILQ35_RS23425	4594715..4595500	-	786	WP_000207556.1	putative lateral flagellar export/assembly protein LafU	-
ILQ35_RS23430	4595472..4597184	+	1713	Protein_4587	flagellar biosynthesis protein FlhA	-
ILQ35_RS23435	4597329..4597826	-	498	Protein_4588	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	gmhA/lpcA / rhs/PAAR	4592843..4614544	21701

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15473.86 Da
• Isoelectric Point: 8.0949

>T176589 WP_001263495.1 NZ_CP062782:c4593241-4592843 [Escherichia coli O157:H7]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDESHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T176589 NZ_CP082835:c4384262-4383987 [Escherichia coli]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATATTTAAAACTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGTAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT176589 WP_000554757.1 NZ_CP062782:c4593537-4593244 [Escherichia coli O157:H7]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT176589 NZ_CP082835:c4383990-4383631 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCTGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Structures

Antitoxin

• AlphaFold DB: S1FJN6