Detailed information of TA system TA176579

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): ralRA/-
• Location: 2355844..2356215
• Replicon: chromosome
• Accession: NZ_CP062782
• Organism: Escherichia coli O157:H7 strain Z869

Toxin (Protein)

• Gene name: ralR
• Uniprot ID: A0A891STD1
• Locus tag: ILQ35_RS11960
• Protein ID: WP_042853000.1
• Coordinates: 2356021..2356215 (-)
• Length: 65 a.a.

Antitoxin (RNA)

Gene name	ralA
Locus tag	-
Coordinates	2355844..2356022 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ILQ35_RS11920	2351286..2351942	-	657	WP_000011656.1	carbon-nitrogen hydrolase family protein	-
ILQ35_RS11925	2352044..2352274	-	231	WP_000916763.1	DNA polymerase III subunit theta	-
ILQ35_RS11930	2352413..2352787	+	375	WP_000168751.1	CopC domain-containing protein YobA	-
ILQ35_RS11935	2352791..2353663	+	873	WP_000879314.1	copper homeostasis membrane protein CopD	-
ILQ35_RS11940	2353676..2354017	+	342	WP_000976483.1	YebY family protein	-
ILQ35_RS11945	2354410..2355486	-	1077	WP_045893699.1	phage integrase Arm DNA-binding domain-containing protein	-
ILQ35_RS11950	2355452..2355733	-	282	WP_001311878.1	excisionase	-
ILQ35_RS11955	2355840..2356028	-	189	WP_001356607.1	DUF1187 family protein	-
-	2355844..2356022	+	179	NuclAT_0	-	Antitoxin
-	2355844..2356022	+	179	NuclAT_0	-	Antitoxin
-	2355844..2356022	+	179	NuclAT_0	-	Antitoxin
-	2355844..2356022	+	179	NuclAT_0	-	Antitoxin
ILQ35_RS11960	2356021..2356215	-	195	WP_042853000.1	type I toxin-antitoxin system endodeoxyribonuclease toxin RalR	Toxin
ILQ35_RS11965	2356272..2357081	-	810	WP_000166313.1	recombination protein RecT	-
ILQ35_RS11970	2357074..2359755	-	2682	WP_045893700.1	exodeoxyribonuclease VIII	-
ILQ35_RS11975	2359856..2360132	-	277	Protein_2335	hypothetical protein	-
ILQ35_RS11980	2360207..2360383	-	177	WP_000245528.1	hypothetical protein	-
ILQ35_RS11985	2360377..2360598	-	222	WP_000560227.1	cell division protein FtsZ	-
ILQ35_RS11990	2361007..2361159	-	153	WP_000379547.1	DUF1391 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2346635..2409305	62670

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endodeoxyribonuclease toxin RalR (6-54)

Sequences

Toxin

Protein

• Length: 65 a.a.
• Molecular weight: 7019.87 Da
• Isoelectric Point: 8.6419

>T176579 WP_042853000.1 NZ_CP062782:c2356215-2356021 [Escherichia coli O157:H7]
MRYDDVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALERWNKRTTGNNNGGVHV

Nucleotide

• Length: 195 bp

>T176579 NZ_CP082835:1952106-1952282 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Nucleotide

• Length: 179 bp

>AT176579 NZ_CP062782:2355844-2356022 [Escherichia coli O157:H7]
GAGGACAGAAGTTTCTCGCAATTAAAATTTATCAGCTTTACTTTCTGCTCTCTGGACACGCCTGCTTCTTTTTTCCCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAATTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCTTTTT
CAATAGTGGCAGTTATTTT

Structures

Antitoxin