Detailed information of TA system TA176553

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 4468669..4469363
• Replicon: chromosome
• Accession: NZ_CP062780
• Organism: Escherichia coli O157:H7 strain Z1769

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: C3TNX7
• Locus tag: ILQ49_RS22550
• Protein ID: WP_001263495.1
• Coordinates: 4468669..4469067 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: ILQ49_RS22555
• Protein ID: WP_000554757.1
• Coordinates: 4469070..4469363 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
-	4464256..4464336	-	81	NuclAT_11	-	-
-	4464256..4464336	-	81	NuclAT_11	-	-
-	4464256..4464336	-	81	NuclAT_11	-	-
-	4464256..4464336	-	81	NuclAT_11	-	-
ILQ49_RS22525	4464932..4465390	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
ILQ49_RS22530	4465651..4467108	+	1458	WP_001293009.1	cytosol nonspecific dipeptidase	-
ILQ49_RS22535	4467165..4467686	-	522	Protein_4411	peptide chain release factor H	-
ILQ49_RS22540	4467685..4467888	-	204	Protein_4412	RNA ligase RtcB family protein	-
ILQ49_RS22545	4468207..4468659	-	453	WP_001059871.1	GNAT family N-acetyltransferase	-
ILQ49_RS22550	4468669..4469067	-	399	WP_001263495.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
ILQ49_RS22555	4469070..4469363	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
ILQ49_RS22560	4469415..4470470	-	1056	WP_001226186.1	DNA polymerase IV	-
ILQ49_RS22565	4470541..4471326	-	786	WP_000207556.1	putative lateral flagellar export/assembly protein LafU	-
ILQ49_RS22570	4471298..4473010	+	1713	Protein_4418	flagellar biosynthesis protein FlhA	-
ILQ49_RS22575	4473155..4473652	-	498	Protein_4419	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	4430516..4474601	44085

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15473.86 Da
• Isoelectric Point: 8.0949

>T176553 WP_001263495.1 NZ_CP062780:c4469067-4468669 [Escherichia coli O157:H7]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDESHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T176553 NZ_CP082833:c401550-401143 [Citrobacter rodentium NBRC 105723 = DSM 16636]
GTGGTCCTGTGGCAATCTGATCTTCGCGTCTCATGGCGCGCGCAGTGGCTGTCGCTACTCCTGCATGGCCTGGTGGCCGC
GGCGATTTTATTAATGCCTTGGCCGTTGAGTTACACGCCGCTGTGGTTGTTATTACTTTCACTGGTCGTGTTTGACTGCG
TTCGCAGTCAGCGGCGAATTAATGCCTGTCAGGGCGAGGTGAAACTGCTGATGGATGGCCGGCTGCACTGGCAGGGGCAG
GACTGGTCGGTTATCAGCGCCCCGTGGATGATGAAAGCCGGGATGATGCTGCGCCTGCGTTCAGACGGCGGTAAACGCCA
GCACCTGTGGATCGCGGCGGACAGCATGGATGAGGCGGAGTGGCGCGATCTCTGCCGCCTGATGCTGCAACCGTCGACGC
AGCGCTAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT176553 WP_000554757.1 NZ_CP062780:c4469363-4469070 [Escherichia coli O157:H7]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT176553 NZ_CP082833:c401797-401531 [Citrobacter rodentium NBRC 105723 = DSM 16636]
ATGGATATTAACAATAAAGCACGTATTCACTGGGCATGCCGTCGCGGTATGCGTGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAATACGACACGTTAAGCGATGCGGAAAAACGCATTTTCGTCCGCCTGCTGGAGTGTGACGATCCAGATT
TATTTAACTGGTTAATGAATCACGGTAAACCGGCAGATGCTGAACTGGAGCAGATGGTACGACTCATCCAGATACGGAAT
CGGGATCGTGGTCCTGTGGCAATCTGA

Structures

Antitoxin

• AlphaFold DB: S1FJN6