Detailed information of TA system TA176543

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 2645885..2646110
• Replicon: chromosome
• Accession: NZ_CP062780
• Organism: Escherichia coli O157:H7 strain Z1769

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: A0A1Q4PF16
• Locus tag: ILQ49_RS13430
• Protein ID: WP_000813258.1
• Coordinates: 2645955..2646110 (+)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	2645885..2645943 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ILQ49_RS13380	2640950..2641192	+	243	WP_000747948.1	hypothetical protein	-
ILQ49_RS13385	2641176..2641601	+	426	WP_000693878.1	Rha family transcriptional regulator	-
ILQ49_RS13390	2641670..2642713	+	1044	WP_001262402.1	hypothetical protein	-
ILQ49_RS13395	2642706..2643167	+	462	WP_000139447.1	replication protein	-
ILQ49_RS13400	2643201..2643917	+	717	WP_000450627.1	DUF1627 domain-containing protein	-
ILQ49_RS13405	2643950..2644231	+	282	WP_000603384.1	DNA-binding protein	-
ILQ49_RS13410	2644228..2644455	+	228	WP_000699809.1	hypothetical protein	-
ILQ49_RS13415	2644448..2644759	+	312	WP_001289673.1	hypothetical protein	-
ILQ49_RS13420	2644887..2645105	+	219	WP_000683609.1	DUF4014 family protein	-
ILQ49_RS13425	2645107..2645664	+	558	WP_000104474.1	DUF551 domain-containing protein	-
-	2645885..2645943	-	59	-	-	Antitoxin
ILQ49_RS13430	2645955..2646110	+	156	WP_000813258.1	type I toxin-antitoxin system Hok family toxin	Toxin
ILQ49_RS13435	2646230..2646574	+	345	WP_000756596.1	YgiW/YdeI family stress tolerance OB fold protein	-
ILQ49_RS13440	2646696..2646968	+	273	WP_000191872.1	hypothetical protein	-
ILQ49_RS13445	2646970..2648019	+	1050	WP_001265229.1	DUF968 domain-containing protein	-
ILQ49_RS13450	2648032..2648337	+	306	WP_001217444.1	RusA family crossover junction endodeoxyribonuclease	-
ILQ49_RS13455	2648400..2648954	+	555	WP_000640035.1	DUF1133 family protein	-
ILQ49_RS13460	2649179..2649376	+	198	WP_000917763.1	hypothetical protein	-
ILQ49_RS13465	2649512..2650225	+	714	WP_000301797.1	helix-turn-helix domain-containing protein	-
ILQ49_RS13480	2650676..2651107	+	432	WP_001302123.1	tellurite resistance protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	nleG7'	2629893..2650225	20332

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5767.95 Da
• Isoelectric Point: 7.7942

>T176543 WP_000813258.1 NZ_CP062780:2645955-2646110 [Escherichia coli O157:H7]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFVDYESEK

Nucleotide

• Length: 156 bp

>T176543 NZ_CP082831:c4234030-4233746 [Escherichia coli]
ATGCAATTTACGGTATACCGCAGTCGCGGCAGGAACGCCGCGTTTCCCTTTGTTATTGATGTTACCAGCGACATTATTGG
TGAGATTAATCGCCGTATCGTTATTCCATTAACACCTATTGAACGATTTAGCCGTATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGATAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACGTTGATTAAATGA

Antitoxin

Nucleotide

• Length: 59 bp

>AT176543 NZ_CP062780:c2645943-2645885 [Escherichia coli O157:H7]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACATGTGTTCAGCATGGATTGAGCCTCA

Structures

Toxin

• AlphaFold DB: A0A1Q4PF16

Antitoxin