Detailed information of TA system TA176517

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 4495557..4496251
• Replicon: chromosome
• Accession: NZ_CP062778
• Organism: Escherichia coli O157:H7 strain Z1836

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: C3TNX7
• Locus tag: ILQ64_RS22795
• Protein ID: WP_001263495.1
• Coordinates: 4495557..4495955 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: ILQ64_RS22800
• Protein ID: WP_000554757.1
• Coordinates: 4495958..4496251 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
-	4491144..4491224	-	81	NuclAT_11	-	-
-	4491144..4491224	-	81	NuclAT_11	-	-
-	4491144..4491224	-	81	NuclAT_11	-	-
-	4491144..4491224	-	81	NuclAT_11	-	-
ILQ64_RS22770	4491820..4492278	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
ILQ64_RS22775	4492539..4493996	+	1458	WP_001293009.1	cytosol nonspecific dipeptidase	-
ILQ64_RS22780	4494053..4494574	-	522	Protein_4462	peptide chain release factor H	-
ILQ64_RS22785	4494573..4494776	-	204	Protein_4463	RNA ligase RtcB family protein	-
ILQ64_RS22790	4495095..4495547	-	453	WP_001059871.1	GNAT family N-acetyltransferase	-
ILQ64_RS22795	4495557..4495955	-	399	WP_001263495.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
ILQ64_RS22800	4495958..4496251	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
ILQ64_RS22805	4496303..4497358	-	1056	WP_001226186.1	DNA polymerase IV	-
ILQ64_RS22810	4497429..4498214	-	786	WP_000207556.1	putative lateral flagellar export/assembly protein LafU	-
ILQ64_RS22815	4498186..4499898	+	1713	Protein_4469	flagellar biosynthesis protein FlhA	-
ILQ64_RS22820	4500043..4500540	-	498	Protein_4470	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15473.86 Da
• Isoelectric Point: 8.0949

>T176517 WP_001263495.1 NZ_CP062778:c4495955-4495557 [Escherichia coli O157:H7]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDESHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T176517 NZ_CP082827:3275779-3276165 [Escherichia coli]
ATGGGTGTGTATAAGGCAAGACGATTCAGCCAAAGCACGAAAAAACTTGGTATTCACGATAAAGTTCTCATGGCTGCCGC
TGAAGAAGTGATGCAGGGCATATGGGAGGCTGATTTAGGTAGCGGTGTCATCAAAAAACGTTTACCCCTCCAACAAGGAA
AGAGTGGTGGAGCCAGAACAATTATCTTTTTTAAATCAGCAAATCATGTGTTTTTTTATGATGGTTGGTCCAAGAGTGGT
CTAAGCAGTAAAGGAACCAAAGAAATCGAAGATGATGAACTGGCGGCCTACAAAAAAATGGCAAATGCATTTCTGGCCTT
TAGCAATAAGCAAATAGAGGATTTGATCGAAACTGGCTTTTTGATTGAGGTGAAAAATGAGCGATAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT176517 WP_000554757.1 NZ_CP062778:c4496251-4495958 [Escherichia coli O157:H7]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT176517 NZ_CP082827:3276155-3276484 [Escherichia coli]
ATGAGCGATAAATTACAAATGATGCTGGAAGATGCAAAGGAACTGAATGCACTGGGCCTGATGTCTGATAGTGAAGTTGA
TGCCATTGCCGTCATGGTCAAAGCTCGTGAAGTCAGCGCCCGTATAGCCAAAGTCAAATCAATGACGGGTGAAGAGATCA
AAGCCATCCGCACCCGATACGGCATGTCACAATCCGTGCTGGCACATACAATGGGAATGTCAAAAGAAAGTGTCTCGAAA
TGGGAACGAAATGAAATAAAACCGAGCGGCCCGGCACTACGTATTTTGAATACATTGGCAATAAAAGGACCGGAAGTTTT
TGCAAAGTAA

Structures

Antitoxin

• AlphaFold DB: S1FJN6