Detailed information of TA system TA176512

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 3544432..3544657
• Replicon: chromosome
• Accession: NZ_CP062778
• Organism: Escherichia coli O157:H7 strain Z1836

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: -
• Locus tag: ILQ64_RS18200
• Protein ID: WP_000813263.1
• Coordinates: 3544432..3544587 (-)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	3544599..3544657 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ILQ64_RS18165	3539886..3540599	-	714	WP_000261909.1	helix-turn-helix domain-containing protein	-
ILQ64_RS18170	3540737..3540933	-	197	Protein_3558	TrmB family transcriptional regulator	-
ILQ64_RS18175	3541220..3542038	-	819	WP_000265265.1	CPBP family intramembrane metalloprotease	-
ILQ64_RS18180	3542190..3542561	-	372	WP_000090264.1	antitermination protein	-
ILQ64_RS18185	3542551..3542922	-	372	WP_001217436.1	RusA family crossover junction endodeoxyribonuclease	-
ILQ64_RS18190	3542935..3543984	-	1050	WP_001265172.1	DUF968 domain-containing protein	-
ILQ64_RS18195	3543986..3544264	-	279	WP_001341388.1	hypothetical protein	-
ILQ64_RS18200	3544432..3544587	-	156	WP_000813263.1	type I toxin-antitoxin system toxin HokD	Toxin
-	3544599..3544657	+	59	-	-	Antitoxin
ILQ64_RS18205	3545192..3545965	+	774	WP_000160654.1	alpha/beta hydrolase	-
ILQ64_RS18210	3546317..3546730	-	414	WP_001151233.1	DUF977 family protein	-
ILQ64_RS18215	3546746..3547516	-	771	WP_000450992.1	DUF1627 domain-containing protein	-
ILQ64_RS18220	3547538..3548284	-	747	WP_000788751.1	ATP-binding protein	-
ILQ64_RS18225	3548291..3549400	-	1110	WP_021499850.1	putative primosomal protein I	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5722.86 Da
• Isoelectric Point: 6.1531

>T176512 WP_000813263.1 NZ_CP062778:c3544587-3544432 [Escherichia coli O157:H7]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRVRTGQTEVAVFTAYEPEE

Nucleotide

• Length: 156 bp

>T176512 NZ_CP082827:2530361-2531683 [Escherichia coli]
ATGCCTAAACTTGTCACTTGGATGAACAACCAGCGGGTAGGCGAGTTAACGAAGTTAGCCAATGGCGCGCACACCTTTAA
GTATGCACCGGAGTGGTTAGCCAACCGTTATGCCAGACCGTTATCACTTTCGCTGCCATTGCAGAGGGGGAATATCACCT
CTGATGCCGTATTTAACTTCTTCGATAACCTGTTACCCGATAGCCCGATTGTACGTGACCGGATCGTTAAACGTTATCAT
GCCAAATCCAGACAACCGTTTGATTTATTGTCAGAAATAGGGCGAGACAGCGTTGGTGCCGTGACGTTAATACCCGAAGA
CGAAACCGTAACGCATCCGATAATGGCATGGGAAAAGCTTACTGAAGCCAGACTTGAAGAAGTATTAACGGCTTATAAAG
CAGATATCCCTCTGGGCATGATTAGAGAAGAAAATGACTTTCGCATCTCGGTTGCTGGCGCGCAGGAGAAGACAGCGCTG
CTCAGAATAGGCAATGACTGGTGCATTCCGAAAGGAATAACGCCGACGACGCACATCATTAAATTACCGATTGGCGAAAT
CAGGCAGCCCAATGCGACGCTCGATCTCAGCCAAAGCGTTGATAATGAGTATTACTGTCTGCTGCTGGCGAAAGAACTTG
GGTTGAATGTGCCGGACGCAGAAATCATTAAAGCGGGAAATGTGCGCGCGTTAGCGGTCGAACGTTTTGACAGGCGTTGG
AATGCTGAGCGAACGGTTTTACTTCGCTTGCCACAGGAGGATATGTGTCAGACATTCGGTTTACCTTCATCGGTGAAATA
TGAATCAGATGGAGGCCCAGGCATCGCGCGGATCATGGCTTTTTTGATGGGGTCCAGCGAGGCGCTGAAAGATCGCTATG
ATTTTATGAAGTTTCAGGTGTTCCAGTGGTTGATTGGCGCAACGGATGGCCATGCAAAAAACTTCTCCGTATTTATTCAG
GCTGGCGGCAGTTATCGACTCACGCCATTTTACGACATCATTTCAGCATTTCCGGTATTGGGTGGCGCAGGAATACACAT
CAGCGATCTCAAACTGGCAATGGGGCTTAACGCATCCAAAGGCAAAAAAACGGCAATCGATAAAATTTATCCGCGACATT
TTCTGGCGACAGCAAAGGTGCTGAAATTTCCGGAAGTGCAGATGCATGAAATCCTGAGTGACTTTGCCAGAATGATTCCG
GCAGCACTGGATAACGTGAAGACTTCATTACCGACAGATTTTCCGGAGAACGTGGTGACGGCAGTTGAAACCAATGTGTT
GAGGTTGCACCGTCGCTTAAGCCGAGAATACGGTATTAAGTAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT176512 NZ_CP062778:3544599-3544657 [Escherichia coli O157:H7]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTAGATTTGCCGTTCATAGATTGAGCCTCA

Structures

Antitoxin