Detailed information of TA system TA176509

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 2976319..2976544
• Replicon: chromosome
• Accession: NZ_CP062778
• Organism: Escherichia coli O157:H7 strain Z1836

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: A0A1Q4PF16
• Locus tag: ILQ64_RS15000
• Protein ID: WP_000813258.1
• Coordinates: 2976319..2976474 (-)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	2976486..2976544 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ILQ64_RS14950	2971322..2971753	-	432	WP_001302123.1	tellurite resistance protein	-
ILQ64_RS14965	2972204..2972917	-	714	WP_000301797.1	helix-turn-helix domain-containing protein	-
ILQ64_RS14970	2973053..2973250	-	198	WP_000917763.1	hypothetical protein	-
ILQ64_RS14975	2973475..2974029	-	555	WP_000640035.1	DUF1133 family protein	-
ILQ64_RS14980	2974092..2974397	-	306	WP_001217444.1	RusA family crossover junction endodeoxyribonuclease	-
ILQ64_RS14985	2974410..2975459	-	1050	WP_001265229.1	DUF968 domain-containing protein	-
ILQ64_RS14990	2975461..2975733	-	273	WP_000191872.1	hypothetical protein	-
ILQ64_RS14995	2975855..2976199	-	345	WP_000756596.1	YgiW/YdeI family stress tolerance OB fold protein	-
ILQ64_RS15000	2976319..2976474	-	156	WP_000813258.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	2976486..2976544	+	59	-	-	Antitoxin
ILQ64_RS15005	2976765..2977322	-	558	WP_000104474.1	DUF551 domain-containing protein	-
ILQ64_RS15010	2977324..2977542	-	219	WP_000683609.1	DUF4014 family protein	-
ILQ64_RS15015	2977670..2977981	-	312	WP_001289673.1	hypothetical protein	-
ILQ64_RS15020	2977974..2978201	-	228	WP_000699809.1	hypothetical protein	-
ILQ64_RS15025	2978198..2978479	-	282	WP_000603384.1	DNA-binding protein	-
ILQ64_RS15030	2978512..2979187	-	676	Protein_2942	DUF1627 domain-containing protein	-
ILQ64_RS15035	2979221..2979682	-	462	WP_000139447.1	replication protein	-
ILQ64_RS15040	2979675..2980718	-	1044	WP_001262402.1	hypothetical protein	-
ILQ64_RS15045	2980787..2981212	-	426	WP_206356580.1	Rha family transcriptional regulator	-
ILQ64_RS15050	2981196..2981438	-	243	WP_000747948.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	nleG7' / paa	2939215..3039028	99813

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5767.95 Da
• Isoelectric Point: 7.7942

>T176509 WP_000813258.1 NZ_CP062778:c2976474-2976319 [Escherichia coli O157:H7]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFVDYESEK

Nucleotide

• Length: 156 bp

>T176509 NZ_CP082827:c849253-848876 [Escherichia coli]
ATGAAAACATTACCCGACACACACGTACGGGCGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTATGGCCTCACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGGATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGTACCGACCAGCCGGGATTC
AGCGCAGGAGCCCCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGGAA
CAATTACAGAATGGTAAATAACATTACCCAGGGCAAGCATCCGGAGGCAAAACGATGA

Antitoxin

Nucleotide

• Length: 59 bp

>AT176509 NZ_CP062778:2976486-2976544 [Escherichia coli O157:H7]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACATGTGTTCAGCATGGATTGAGCCTCA

Structures

Toxin

• AlphaFold DB: A0A1Q4PF16

Antitoxin