Detailed information of TA system TA176506

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 2608903..2609128
• Replicon: chromosome
• Accession: NZ_CP062778
• Organism: Escherichia coli O157:H7 strain Z1836

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: S1ELB5
• Locus tag: ILQ64_RS13210
• Protein ID: WP_000813254.1
• Coordinates: 2608973..2609128 (+)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	2608903..2608961 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ILQ64_RS13165	2604156..2605109	+	954	WP_000095667.1	helix-turn-helix domain-containing protein	-
ILQ64_RS13170	2605116..2605856	+	741	WP_206356613.1	ATP-binding protein	-
ILQ64_RS13175	2605886..2606656	+	771	WP_000450888.1	DUF1627 domain-containing protein	-
ILQ64_RS13180	2606672..2607067	+	396	WP_001118161.1	DUF977 family protein	-
ILQ64_RS13185	2607124..2607480	+	357	WP_001302146.1	hypothetical protein	-
ILQ64_RS13190	2607529..2607741	+	213	WP_000063625.1	hypothetical protein	-
ILQ64_RS13195	2607777..2608148	+	372	WP_000137941.1	hypothetical protein	-
ILQ64_RS13200	2608145..2608507	+	363	WP_000610379.1	DUF551 domain-containing protein	-
ILQ64_RS13205	2608623..2608727	+	105	WP_001278450.1	hypothetical protein	-
-	2608903..2608961	-	59	-	-	Antitoxin
ILQ64_RS13210	2608973..2609128	+	156	WP_000813254.1	type I toxin-antitoxin system toxin HokD	Toxin
ILQ64_RS13215	2609407..2609574	+	168	WP_000998188.1	hypothetical protein	-
ILQ64_RS13220	2609640..2609918	+	279	WP_001304183.1	hypothetical protein	-
ILQ64_RS13225	2609920..2610969	+	1050	WP_001302870.1	DUF968 domain-containing protein	-
ILQ64_RS13230	2610982..2611356	+	375	WP_000904137.1	RusA family crossover junction endodeoxyribonuclease	-
ILQ64_RS13235	2611353..2612174	+	822	WP_000762904.1	antitermination protein	-
ILQ64_RS13240	2612401..2612598	+	198	WP_000917735.1	hypothetical protein	-
ILQ64_RS13245	2612749..2613807	+	1059	WP_000483497.1	site-specific DNA-methyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	nleA/espI	2595401..2646385	50984

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5736.89 Da
• Isoelectric Point: 6.1531

>T176506 WP_000813254.1 NZ_CP062778:2608973-2609128 [Escherichia coli O157:H7]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFTAYEPEE

Nucleotide

• Length: 156 bp

>T176506 NZ_CP082827:677862-678176 [Escherichia coli]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTGGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAATCATTAAAAGCAGTATTCCCATCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTCGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGCCGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin

Nucleotide

• Length: 59 bp

>AT176506 NZ_CP062778:c2608961-2608903 [Escherichia coli O157:H7]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACGTGTGTAGAGCATAGATATGGCCTCA

Structures

Toxin

• AlphaFold DB: A0A4P7TMW0

Antitoxin