Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 739582..740309 Replicon chromosome
Accession NZ_CP062778
Organism Escherichia coli O157:H7 strain Z1836

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag ILQ64_RS03630 Protein ID WP_000550189.1
Coordinates 739582..739896 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag ILQ64_RS03635 Protein ID WP_000560249.1
Coordinates 739893..740309 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ILQ64_RS03610 735740..736726 - 987 WP_000617675.1 Gfo/Idh/MocA family oxidoreductase -
ILQ64_RS03615 736805..737497 - 693 WP_000942543.1 vancomycin high temperature exclusion protein -
ILQ64_RS03620 737574..738077 - 504 WP_001295542.1 M48 family metallopeptidase -
ILQ64_RS03625 738162..739298 + 1137 WP_000018678.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
ILQ64_RS03630 739582..739896 + 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
ILQ64_RS03635 739893..740309 + 417 WP_000560249.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
ILQ64_RS03640 740354..742372 - 2019 WP_000121479.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
ILQ64_RS03645 742898..745249 - 2352 WP_000695517.1 alpha-glucosidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Antitoxin HigA

(1-138)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T176495 WP_000550189.1 NZ_CP062778:739582-739896 [Escherichia coli O157:H7]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

>T176495 NZ_CP082824:4225393-4225545 [Escherichia coli]
ATGAAGCAGCATAAGGCGATGATTGTCGCCCTGATCGTCATCTGTATCACCGCCGTAGTGGCGGCGCTGGTAACGAGAAA
AGACCTCTGTGAGGTTCACATCCGAACTGGCCAGACGGAGGTTGCTGTTTTCACGGCTTACGAATCCGAGTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15016.48 Da        Isoelectric Point: 4.5805

>AT176495 WP_000560249.1 NZ_CP062778:739893-740309 [Escherichia coli O157:H7]
MIAIADILHAGEKLTAVAPFLAGIQNEEQYIQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp

>AT176495 NZ_CP082824:c4225344-4225287 [Escherichia coli]
GTTCTGCATATAGGGGGCCTCGGGTTGATGGTAAAATATCACTCGGGGCTTTTCTCTA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References