Detailed information of TA system TA176494

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 696745..697544
• Replicon: chromosome
• Accession: NZ_CP062778
• Organism: Escherichia coli O157:H7 strain Z1836

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: V0SSH7
• Locus tag: ILQ64_RS03410
• Protein ID: WP_000347273.1
• Coordinates: 696745..697209 (-)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: C3ST72
• Locus tag: ILQ64_RS03415
• Protein ID: WP_001302819.1
• Coordinates: 697209..697544 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ILQ64_RS03380	691746..692180	-	435	WP_000948824.1	PTS sugar transporter subunit IIA	-
ILQ64_RS03385	692198..693076	-	879	WP_001295548.1	PTS system mannose/fructose/sorbose family transporter subunit IID	-
ILQ64_RS03390	693066..693845	-	780	WP_000406214.1	PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC	-
ILQ64_RS03395	693856..694329	-	474	WP_001301475.1	PTS N-acetylgalactosamine transporter subunit IIB	-
ILQ64_RS03400	694352..695632	-	1281	WP_000681927.1	tagatose-bisphosphate aldolase subunit KbaZ	-
ILQ64_RS03405	695881..696690	+	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
ILQ64_RS03410	696745..697209	-	465	WP_000347273.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
ILQ64_RS03415	697209..697544	-	336	WP_001302819.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
ILQ64_RS03420	697693..699264	-	1572	WP_001273776.1	galactarate dehydratase	-
ILQ64_RS03425	699639..700973	+	1335	WP_000599636.1	galactarate/glucarate/glycerate transporter GarP	-
ILQ64_RS03430	700989..701759	+	771	WP_001058227.1	2-dehydro-3-deoxyglucarate aldolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17836.25 Da
• Isoelectric Point: 9.6924

>T176494 WP_000347273.1 NZ_CP062778:c697209-696745 [Escherichia coli O157:H7]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Nucleotide

• Length: 465 bp

>T176494 NZ_CP082824:c4188842-4188528 [Escherichia coli]
ATGCAATTTACGGTATACCGCAGTCGCGGCAGGAACGCCGCGTTTCCCTTTGTTATTGATGTTACCAGCGACATTATTGG
TGAGATTAATCGCCGTATCGTTATTCCATTAACACCTATTGAACGATTTAGCCGTATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGGTAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACGTTGATTAAAGGAGCATTAGATTTTATGCTCGACGGGATTTAA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12388.97 Da
• Isoelectric Point: 4.8616

>AT176494 WP_001302819.1 NZ_CP062778:c697544-697209 [Escherichia coli O157:H7]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDTDIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT176494 NZ_CP082824:c4189078-4188845 [Escherichia coli]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACA
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Structures

Toxin

• AlphaFold DB: V0SSH7