Detailed information of TA system TA176478

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 4456991..4457685
• Replicon: chromosome
• Accession: NZ_CP062774
• Organism: Escherichia coli O157:H7 strain Z563

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: C3TNX7
• Locus tag: ILQ31_RS22470
• Protein ID: WP_001263495.1
• Coordinates: 4456991..4457389 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: ILQ31_RS22475
• Protein ID: WP_000554757.1
• Coordinates: 4457392..4457685 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
-	4452578..4452658	-	81	NuclAT_11	-	-
-	4452578..4452658	-	81	NuclAT_11	-	-
-	4452578..4452658	-	81	NuclAT_11	-	-
-	4452578..4452658	-	81	NuclAT_11	-	-
ILQ31_RS22445	4453254..4453712	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
ILQ31_RS22450	4453973..4455430	+	1458	WP_001293009.1	cytosol nonspecific dipeptidase	-
ILQ31_RS22455	4455487..4456008	-	522	Protein_4395	peptide chain release factor H	-
ILQ31_RS22460	4456007..4456210	-	204	Protein_4396	RNA ligase RtcB family protein	-
ILQ31_RS22465	4456529..4456981	-	453	WP_001059871.1	GNAT family N-acetyltransferase	-
ILQ31_RS22470	4456991..4457389	-	399	WP_001263495.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
ILQ31_RS22475	4457392..4457685	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
ILQ31_RS22480	4457737..4458792	-	1056	WP_001226186.1	DNA polymerase IV	-
ILQ31_RS22485	4458863..4459648	-	786	WP_000207556.1	putative lateral flagellar export/assembly protein LafU	-
ILQ31_RS22490	4459620..4461332	+	1713	Protein_4402	flagellar biosynthesis protein FlhA	-
ILQ31_RS22495	4461477..4461974	-	498	Protein_4403	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15473.86 Da
• Isoelectric Point: 8.0949

>T176478 WP_001263495.1 NZ_CP062774:c4457389-4456991 [Escherichia coli O157:H7]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDESHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T176478 NZ_CP082823:c5775949-5775539 [Pseudomonas aeruginosa]
ATGCTCAAGTTCATGCTTGACACCAACATCTGCATCTTCACCATCAAAAACCGTCCGCAAGAGGTGCGCGAGGCGTTCCT
GCGCCACCACGACCAGATGTGCATCAGCACCGTGACGCTGATGGAGCTGATCTACGGCGCAGAGAAGTCCTCGAACCCCT
CGCGCAATCTTGCAGACGTGGAAGGCTTCGCTGCTCGTCTGGAGGTGCTCAAGTACGACCAGGAAGCTGCCGCCCACACC
GGCCAGCTCCGCGCTGAACTGGCCCGTCTCGGCAAGCAAATCGGCCCCTATGACCAGATGATCGCCGGGCACGCCCGCTC
TCAGGGCCTGATCGTGGTCACCAATAACCGCCGCGAGTTCGACCGCGTACCCGGCTTGAGAGTTGAGGATTGGGTCACCC
CAATCGTATAG

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT176478 WP_000554757.1 NZ_CP062774:c4457685-4457392 [Escherichia coli O157:H7]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT176478 NZ_CP082823:c5776179-5775949 [Pseudomonas aeruginosa]
GTGGAACAAGGTTCTGTTTTTCAGAGCAATCGTAGCCAGGCCGTAAGACTGCCCAAGGCCGTCGCGCTTCCTGATGACGT
TAAGCGTGTGGATGTGGTCGCTGTGGGGCGCGCCAGAATCATCACGCCTGCCGGTGAGTCTTGGGATAGCTGGTTCGATG
GCGAGGGCGTTACTGCCGACTTTATGGCAGAGCGTGAACAGCCTGCCGATCAGGAGCGCGAGACTTTCTGA

Structures

Antitoxin

• AlphaFold DB: S1FJN6