Detailed information of TA system TA176473

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 3571698..3571923
• Replicon: chromosome
• Accession: NZ_CP062774
• Organism: Escherichia coli O157:H7 strain Z563

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: -
• Locus tag: ILQ31_RS18360
• Protein ID: WP_000813263.1
• Coordinates: 3571698..3571853 (-)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	3571865..3571923 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ILQ31_RS18325	3567152..3567865	-	714	WP_000261909.1	helix-turn-helix domain-containing protein	-
ILQ31_RS18330	3568003..3568199	-	197	Protein_3587	TrmB family transcriptional regulator	-
ILQ31_RS18335	3568486..3569304	-	819	WP_000265265.1	CPBP family intramembrane metalloprotease	-
ILQ31_RS18340	3569456..3569827	-	372	WP_000090264.1	antitermination protein	-
ILQ31_RS18345	3569817..3570188	-	372	WP_001217436.1	RusA family crossover junction endodeoxyribonuclease	-
ILQ31_RS18350	3570201..3571250	-	1050	WP_001265172.1	DUF968 domain-containing protein	-
ILQ31_RS18355	3571252..3571530	-	279	WP_001341388.1	hypothetical protein	-
ILQ31_RS18360	3571698..3571853	-	156	WP_000813263.1	type I toxin-antitoxin system toxin HokD	Toxin
-	3571865..3571923	+	59	-	-	Antitoxin
ILQ31_RS18365	3572458..3573231	+	774	WP_000160654.1	alpha/beta hydrolase	-
ILQ31_RS18370	3573583..3573996	-	414	WP_001151233.1	DUF977 family protein	-
ILQ31_RS18375	3574012..3574782	-	771	WP_000450992.1	DUF1627 domain-containing protein	-
ILQ31_RS18380	3574804..3575550	-	747	WP_000788751.1	ATP-binding protein	-
ILQ31_RS18385	3575557..3576648	-	1092	WP_001205823.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5722.86 Da
• Isoelectric Point: 6.1531

>T176473 WP_000813263.1 NZ_CP062774:c3571853-3571698 [Escherichia coli O157:H7]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRVRTGQTEVAVFTAYEPEE

Nucleotide

• Length: 156 bp

>T176473 NZ_CP082823:c4928652-4927897 [Pseudomonas aeruginosa]
GTGAGCGAGATCTGGCGACAGTGCAAGGGTGAGCGGCATGTCCGACCGCTCCAGGGGCGGCTGGTGCGGTTGGTGGAGAG
CCAGGAGCAGGTGGCGACCCTGCAATTGGTCGACACCCTGGAAGAACAGGCGTTGCTCGAGGAACTGCTCGAGTCGAGCA
AGCCGCCGGTGCCGGCGGATGCCGAGCCATTGCATTACCTGTTGAAGACCCCGTTCCGTTATCCGCCCTTGCGTTGGGGG
TCGCGGTTTGGCCGTCGTCACGAGCCAAGCCTGTTCTACGCCGCGTTGAAGCTGGAAACGGCCATGGCCGAAAGCGCGTA
TTACCGTTGTGTCCTGTGGAGTGGCATGGCGGTGCCGCCACCGAGCGGGAGGATTCTGTCCGAGCACGCTTCGTTCGAGG
CGGGCTGGAAGGTGGAGCGGGGAATCCGTCTGCAGGCGCCGCCTTTCTCCGATCACGAGGCTGCGCTGACCGACATCGCC
GACTACCGCGCTCCCCAGGAACTGGGGAGTGCCATGCGCAGCGCCGGGGTGCAGGCATTCGAATACCGTTCGGCGCGTTG
CCCGGAGCGCGGCTGCAATGTCGCCCTGTTCACGCCCGCGGCCTTCACCGAAAAGCGTCCTCGCAACCTGACGCCCTGGC
TGTGCGAAACCACGGCCGGTTACGTGGCCTTCAAGCCGGCGCATGTGCCGGGGTCGCCGAAAATCTTCTCCTGGGAGCTG
TTCCTGGTGGACGGAAAGTTGCCGCATCCGGCGTGA

Antitoxin

Nucleotide

• Length: 59 bp

>AT176473 NZ_CP062774:3571865-3571923 [Escherichia coli O157:H7]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTAGATTTGCCGTTCATAGATTGAGCCTCA

Structures

Antitoxin