Detailed information of TA system TA176462

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 2675827..2676052
• Replicon: chromosome
• Accession: NZ_CP062774
• Organism: Escherichia coli O157:H7 strain Z563

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: A0A1Q4PF16
• Locus tag: ILQ31_RS13470
• Protein ID: WP_000813258.1
• Coordinates: 2675827..2675982 (-)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	2675994..2676052 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ILQ31_RS13420	2670830..2671261	-	432	WP_001302123.1	tellurite resistance protein	-
ILQ31_RS13435	2671712..2672425	-	714	WP_000301797.1	helix-turn-helix domain-containing protein	-
ILQ31_RS13440	2672561..2672758	-	198	WP_000917763.1	hypothetical protein	-
ILQ31_RS13445	2672983..2673537	-	555	WP_000640035.1	DUF1133 family protein	-
ILQ31_RS13450	2673600..2673905	-	306	WP_001217444.1	RusA family crossover junction endodeoxyribonuclease	-
ILQ31_RS13455	2673918..2674967	-	1050	WP_001265229.1	DUF968 domain-containing protein	-
ILQ31_RS13460	2674969..2675241	-	273	WP_000191872.1	hypothetical protein	-
ILQ31_RS13465	2675363..2675707	-	345	WP_000756596.1	YgiW/YdeI family stress tolerance OB fold protein	-
ILQ31_RS13470	2675827..2675982	-	156	WP_000813258.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	2675994..2676052	+	59	-	-	Antitoxin
ILQ31_RS13475	2676273..2676830	-	558	WP_000104474.1	DUF551 domain-containing protein	-
ILQ31_RS13480	2676832..2677050	-	219	WP_000683609.1	DUF4014 family protein	-
ILQ31_RS13485	2677178..2677489	-	312	WP_001289673.1	hypothetical protein	-
ILQ31_RS13490	2677482..2677709	-	228	WP_000699809.1	hypothetical protein	-
ILQ31_RS13495	2677706..2677987	-	282	WP_000603384.1	DNA-binding protein	-
ILQ31_RS13500	2678020..2678736	-	717	WP_000450627.1	DUF1627 domain-containing protein	-
ILQ31_RS13505	2678770..2679231	-	462	WP_000139447.1	replication protein	-
ILQ31_RS13510	2679224..2680267	-	1044	WP_001262402.1	hypothetical protein	-
ILQ31_RS13515	2680336..2680761	-	426	WP_000693878.1	Rha family transcriptional regulator	-
ILQ31_RS13520	2680745..2680987	-	243	WP_000747948.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	nleC / nleG7' / paa	2592821..2737727	144906

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5767.95 Da
• Isoelectric Point: 7.7942

>T176462 WP_000813258.1 NZ_CP062774:c2675982-2675827 [Escherichia coli O157:H7]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFVDYESEK

Nucleotide

• Length: 156 bp

>T176462 NZ_CP082822:2800295-2800870 [Pseudomonas aeruginosa]
ATGAGGCATTCCCCATTCACCGCCGTCTACGACGCGTGCGTTCTATATCCCGCGCCACTGCGGGATTTCCTGATGTGGCT
CGGCCTGTCTGGCCGCTTCCGGGCGCGGTGGAGCCAAGCCATTCATGAGGAGTGGAAACGCAATCTGTTGATCAACCGCC
CCGATCTCACCCGGGTTCAGGTCGACAGGACGTCGGATCTCATGGACAGGGCCATTCCGGACGGCTTGGTGGAGGGCTAC
GAAGCGCTCGTGGCAGGCCTGACATTACCCGATCCGAACGATCGGCACGTCCTGGCTGCGGCGATTCGCTGCGGTGCGAG
CGTGATTGTGACGTTCAACGAACGTGATTTCCCGAACGATCTGCTGGCTCCGTACGGCATCGAATCGCAGCACCCCGATG
AGTTTGTGGACAACCTGCTGGATCTGGATGCGGCCGCCGTAGTGTCGGCTGCGCAGCGCCAGCGTGCCCAACTCAAGCAT
CCGCCGATCGATGTGGACCGCTATCTCGAAATCCTGCTGCGCCAAGGCCTTGTGCAAACGACCAAGGTGCTGGCGACCTA
TCGCACCATTCTCTGA

Antitoxin

Nucleotide

• Length: 59 bp

>AT176462 NZ_CP062774:2675994-2676052 [Escherichia coli O157:H7]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACATGTGTTCAGCATGGATTGAGCCTCA

Structures

Toxin

• AlphaFold DB: A0A1Q4PF16

Antitoxin