Detailed information of TA system TA176435

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 3621341..3621566
• Replicon: chromosome
• Accession: NZ_CP062771
• Organism: Escherichia coli O157:H7 strain Z570

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: -
• Locus tag: ILQ32_RS18700
• Protein ID: WP_000813263.1
• Coordinates: 3621341..3621496 (-)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	3621508..3621566 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ILQ32_RS18665	3616795..3617508	-	714	WP_000261909.1	helix-turn-helix domain-containing protein	-
ILQ32_RS18670	3617646..3617842	-	197	Protein_3655	TrmB family transcriptional regulator	-
ILQ32_RS18675	3618129..3618947	-	819	WP_000265265.1	CPBP family intramembrane metalloprotease	-
ILQ32_RS18680	3619099..3619470	-	372	WP_000090264.1	antitermination protein	-
ILQ32_RS18685	3619460..3619831	-	372	WP_032283490.1	RusA family crossover junction endodeoxyribonuclease	-
ILQ32_RS18690	3619844..3620893	-	1050	WP_206338758.1	DUF968 domain-containing protein	-
ILQ32_RS18695	3620895..3621173	-	279	WP_001341388.1	hypothetical protein	-
ILQ32_RS18700	3621341..3621496	-	156	WP_000813263.1	type I toxin-antitoxin system toxin HokD	Toxin
-	3621508..3621566	+	59	-	-	Antitoxin
ILQ32_RS18705	3622101..3622874	+	774	WP_000160654.1	alpha/beta hydrolase	-
ILQ32_RS18710	3623226..3623639	-	414	WP_001151233.1	DUF977 family protein	-
ILQ32_RS18715	3623655..3624425	-	771	WP_000450992.1	DUF1627 domain-containing protein	-
ILQ32_RS18720	3624447..3625193	-	747	WP_000788751.1	ATP-binding protein	-
ILQ32_RS18725	3625200..3626291	-	1092	WP_001205823.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5722.86 Da
• Isoelectric Point: 6.1531

>T176435 WP_000813263.1 NZ_CP062771:c3621496-3621341 [Escherichia coli O157:H7]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRVRTGQTEVAVFTAYEPEE

Nucleotide

• Length: 156 bp

>T176435 NZ_CP082815:c1925841-1925689 [Staphylococcus aureus]
ATGTCTAAAGACAAAGATCCAAAATTAAATTATCATGAAGAAGAAAACAGTATGGTAACTGATTTTGAAGATTTAAAAGA
ATTAGGTAAAGAAATGGAACAAATTTCTGATCAAAATGATCAAGAAAAGAATTCTGAAGAAGACAGTCAGTAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT176435 NZ_CP062771:3621508-3621566 [Escherichia coli O157:H7]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTAGATTTGCCGTTCATAGATTGAGCCTCA

Structures

Antitoxin