Detailed information of TA system TA176431

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 2642177..2642402
• Replicon: chromosome
• Accession: NZ_CP062771
• Organism: Escherichia coli O157:H7 strain Z570

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: A0A1Q4PF16
• Locus tag: ILQ32_RS13425
• Protein ID: WP_000813258.1
• Coordinates: 2642247..2642402 (+)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	2642177..2642235 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ILQ32_RS13375	2637242..2637484	+	243	WP_000747948.1	hypothetical protein	-
ILQ32_RS13380	2637468..2637893	+	426	WP_000693878.1	Rha family transcriptional regulator	-
ILQ32_RS13385	2637962..2639005	+	1044	WP_001262402.1	hypothetical protein	-
ILQ32_RS13390	2638998..2639459	+	462	WP_000139447.1	replication protein	-
ILQ32_RS13395	2639493..2640209	+	717	WP_000450627.1	DUF1627 domain-containing protein	-
ILQ32_RS13400	2640242..2640523	+	282	WP_000603384.1	DNA-binding protein	-
ILQ32_RS13405	2640520..2640747	+	228	WP_000699809.1	hypothetical protein	-
ILQ32_RS13410	2640740..2641051	+	312	WP_001289673.1	hypothetical protein	-
ILQ32_RS13415	2641179..2641397	+	219	WP_000683609.1	DUF4014 family protein	-
ILQ32_RS13420	2641399..2641956	+	558	WP_000104474.1	DUF551 domain-containing protein	-
-	2642177..2642235	-	59	-	-	Antitoxin
ILQ32_RS13425	2642247..2642402	+	156	WP_000813258.1	type I toxin-antitoxin system Hok family toxin	Toxin
ILQ32_RS13430	2642522..2642866	+	345	WP_000756596.1	YgiW/YdeI family stress tolerance OB fold protein	-
ILQ32_RS13435	2642988..2643260	+	273	WP_000191872.1	hypothetical protein	-
ILQ32_RS13440	2643262..2644311	+	1050	WP_001265229.1	DUF968 domain-containing protein	-
ILQ32_RS13445	2644324..2644629	+	306	WP_001217444.1	RusA family crossover junction endodeoxyribonuclease	-
ILQ32_RS13450	2644692..2645246	+	555	WP_000640035.1	DUF1133 family protein	-
ILQ32_RS13455	2645471..2645668	+	198	WP_000917763.1	hypothetical protein	-
ILQ32_RS13460	2645804..2646517	+	714	WP_000301797.1	helix-turn-helix domain-containing protein	-
ILQ32_RS13475	2646968..2647399	+	432	WP_001302123.1	tellurite resistance protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	nleG7' / nleC	2570061..2743002	172941

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5767.95 Da
• Isoelectric Point: 7.7942

>T176431 WP_000813258.1 NZ_CP062771:2642247-2642402 [Escherichia coli O157:H7]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFVDYESEK

Nucleotide

• Length: 156 bp

>T176431 NZ_CP082813:c2084597-2084388 [Staphylococcus aureus]
ATGATACATCAAAATACGATTTACACAGCGGGAATTGAAACAAAAGAACAAGTAAGTCAATTGACAGAACGCATTTCAAA
TATGATAGGTGTTCATCAAGTGAATATTAATATAATTGATGGTCAAGTAACGGTATCGTATGAGACACCAGCAAATTTGA
ATAGTATTGAAAAAGAAATCTATGATGAAGGATACAAAATTGTATTTTAG

Antitoxin

Nucleotide

• Length: 59 bp

>AT176431 NZ_CP062771:c2642235-2642177 [Escherichia coli O157:H7]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACATGTGTTCAGCATGGATTGAGCCTCA

Structures

Toxin

• AlphaFold DB: A0A1Q4PF16

Antitoxin