Detailed information of TA system TA176373

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 4515366..4516060
• Replicon: chromosome
• Accession: NZ_CP062766
• Organism: Escherichia coli O157:H7 strain Z866

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: C3TNX7
• Locus tag: ILQ34_RS22815
• Protein ID: WP_001263495.1
• Coordinates: 4515366..4515764 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: ILQ34_RS22820
• Protein ID: WP_000554757.1
• Coordinates: 4515767..4516060 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
-	4510953..4511033	-	81	NuclAT_11	-	-
-	4510953..4511033	-	81	NuclAT_11	-	-
-	4510953..4511033	-	81	NuclAT_11	-	-
-	4510953..4511033	-	81	NuclAT_11	-	-
ILQ34_RS22790	4511629..4512087	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
ILQ34_RS22795	4512348..4513805	+	1458	WP_001293009.1	cytosol nonspecific dipeptidase	-
ILQ34_RS22800	4513862..4514383	-	522	Protein_4464	peptide chain release factor H	-
ILQ34_RS22805	4514382..4514585	-	204	Protein_4465	RNA ligase RtcB family protein	-
ILQ34_RS22810	4514904..4515356	-	453	WP_001059871.1	GNAT family N-acetyltransferase	-
ILQ34_RS22815	4515366..4515764	-	399	WP_001263495.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
ILQ34_RS22820	4515767..4516060	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
ILQ34_RS22825	4516112..4517167	-	1056	WP_001226186.1	DNA polymerase IV	-
ILQ34_RS22830	4517238..4518023	-	786	WP_000207556.1	putative lateral flagellar export/assembly protein LafU	-
ILQ34_RS22835	4517995..4519707	+	1713	Protein_4471	flagellar biosynthesis protein FlhA	-
ILQ34_RS22840	4519852..4520349	-	498	Protein_4472	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	4477212..4521298	44086

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15473.86 Da
• Isoelectric Point: 8.0949

>T176373 WP_001263495.1 NZ_CP062766:c4515764-4515366 [Escherichia coli O157:H7]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDESHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T176373 NZ_CP082794:21156-21407 [Klebsiella pneumoniae]
ATGAGAAAAAATAAAGGTGATGTGACTTATTTTCTTGAAAAAGAAGGTGACAATTACAGGCTTACAAAAAGGATAAAAGC
CAGAACAAATGTTAAAATCGGGAACAAAACTACGAAAATTACATTGTATGATGCTGTACTGAATGAGAATGAATTACAAC
ATATTGATTTTACGTGTGCCGGATTAAGGGAAGACGATGAGACGCCAGTTAAGAATTTAATTAAGGAGTTTATGTTAAAT
GAAACCCGATAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT176373 WP_000554757.1 NZ_CP062766:c4516060-4515767 [Escherichia coli O157:H7]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT176373 NZ_CP082794:21394-21621 [Klebsiella pneumoniae]
ATGAAACCCGATAATACACCTGAAAATGTAAAAAAATTACGTTTAAAAGCAGGATTAACACAAAAGGAATGCAGTCATAT
TTATGGTGTTGGGCTAAGAACCTGGCAGAAAAAAGAGGAAGTTAATACTCAAAACAGTCAAAGTCTGTCACTGGTTGAGT
TTGAGTTTCTTTTGTTACTTGCAGGAGAACATCCTGAATACGTGCTCTGCAAAAGGGAGTCAAAATAA

Structures

Antitoxin

• AlphaFold DB: S1FJN6