Detailed information of TA system TA176368

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 3627447..3627672
• Replicon: chromosome
• Accession: NZ_CP062766
• Organism: Escherichia coli O157:H7 strain Z866

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: -
• Locus tag: ILQ34_RS18690
• Protein ID: WP_000813263.1
• Coordinates: 3627447..3627602 (-)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	3627614..3627672 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ILQ34_RS18655	3622901..3623614	-	714	WP_000261909.1	helix-turn-helix domain-containing protein	-
ILQ34_RS18660	3623752..3623948	-	197	Protein_3653	TrmB family transcriptional regulator	-
ILQ34_RS18665	3624235..3625053	-	819	WP_000265265.1	CPBP family intramembrane metalloprotease	-
ILQ34_RS18670	3625205..3625576	-	372	WP_000090264.1	antitermination protein	-
ILQ34_RS18675	3625566..3625937	-	372	WP_001217436.1	RusA family crossover junction endodeoxyribonuclease	-
ILQ34_RS18680	3625950..3626999	-	1050	WP_001265172.1	DUF968 domain-containing protein	-
ILQ34_RS18685	3627001..3627279	-	279	WP_001341388.1	hypothetical protein	-
ILQ34_RS18690	3627447..3627602	-	156	WP_000813263.1	type I toxin-antitoxin system toxin HokD	Toxin
-	3627614..3627672	+	59	-	-	Antitoxin
ILQ34_RS18695	3628207..3628980	+	774	WP_000160654.1	alpha/beta hydrolase	-
ILQ34_RS18700	3629332..3629745	-	414	WP_001151233.1	DUF977 family protein	-
ILQ34_RS18705	3629761..3630531	-	771	WP_000450992.1	DUF1627 domain-containing protein	-
ILQ34_RS18710	3630553..3631299	-	747	WP_000788751.1	ATP-binding protein	-
ILQ34_RS18715	3631306..3632397	-	1092	WP_001205823.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5722.86 Da
• Isoelectric Point: 6.1531

>T176368 WP_000813263.1 NZ_CP062766:c3627602-3627447 [Escherichia coli O157:H7]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRVRTGQTEVAVFTAYEPEE

Nucleotide

• Length: 156 bp

>T176368 NZ_CP082791:c3239775-3239392 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT176368 NZ_CP062766:3627614-3627672 [Escherichia coli O157:H7]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTAGATTTGCCGTTCATAGATTGAGCCTCA

Structures

Antitoxin