Detailed information of TA system TA176337

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 4484024..4484718
• Replicon: chromosome
• Accession: NZ_CP062763
• Organism: Escherichia coli O157:H7 strain Z885

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: C3TNX7
• Locus tag: ILQ36_RS22605
• Protein ID: WP_001263495.1
• Coordinates: 4484024..4484422 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: ILQ36_RS22610
• Protein ID: WP_000554757.1
• Coordinates: 4484425..4484718 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
-	4479611..4479691	-	81	NuclAT_11	-	-
-	4479611..4479691	-	81	NuclAT_11	-	-
-	4479611..4479691	-	81	NuclAT_11	-	-
-	4479611..4479691	-	81	NuclAT_11	-	-
ILQ36_RS22580	4480287..4480745	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
ILQ36_RS22585	4481006..4482463	+	1458	WP_001293009.1	cytosol nonspecific dipeptidase	-
ILQ36_RS22590	4482520..4483041	-	522	Protein_4422	peptide chain release factor H	-
ILQ36_RS22595	4483040..4483243	-	204	Protein_4423	RNA ligase RtcB family protein	-
ILQ36_RS22600	4483562..4484014	-	453	WP_001059871.1	GNAT family N-acetyltransferase	-
ILQ36_RS22605	4484024..4484422	-	399	WP_001263495.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
ILQ36_RS22610	4484425..4484718	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
ILQ36_RS22615	4484770..4485825	-	1056	WP_001226186.1	DNA polymerase IV	-
ILQ36_RS22620	4485896..4486681	-	786	WP_000207556.1	putative lateral flagellar export/assembly protein LafU	-
ILQ36_RS22625	4486653..4488365	+	1713	Protein_4429	flagellar biosynthesis protein FlhA	-
ILQ36_RS22630	4488510..4489007	-	498	Protein_4430	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	4445869..4489956	44087

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15473.86 Da
• Isoelectric Point: 8.0949

>T176337 WP_001263495.1 NZ_CP062763:c4484422-4484024 [Escherichia coli O157:H7]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDESHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T176337 NZ_CP082785:c3787506-3787189 [Klebsiella quasipneumoniae]
ATGTTCAGAATGGTTGTGCATGTGGACGTTAAAAAGGAGTTGCAGGCGCTTCCGGCTATTGTCCAGGCGAAGATGATCAG
GCAATTAGACAAGTTACGCCAAAATCCCACCGCGCTGAGAGAACCTGATAGTAAACCGCTTGGACAAGGGCTATTTGAAA
TTCGAGCGTTAGGTTCGGTACAGGGGCGGGGGATCTACGTTTTTCAGCAAAACAAAACGCTCTTTCTGCTACGTGTTTTT
GTCAAGAAAACGCAAAAAACCCCGTTATCGGAAATTCGCCTGGCGCTGAAGCGATTAGAGGAGATGCGCAATGAGTAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT176337 WP_000554757.1 NZ_CP062763:c4484718-4484425 [Escherichia coli O157:H7]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT176337 NZ_CP082785:c3787196-3786909 [Klebsiella quasipneumoniae]
ATGAGTAAAAAGATCATTGACTGGGATACGTTAAAAACAGAACTGCTTAGCCAGCCTGAGGTTCAGGCTGCGTTCGATGC
GCAAGAACGTCAGCACCGATTACGTACTATGCTGGCACAATGGCGCGAGCAGGCGGGGCTGACCCGAGCCCAGGTTGCGC
AGAGGATGGGGGTGAGTGCGCCAACGGTCTCCAGAATGGAAGCCAATATCACCCGCACCAGCCTGGATACCTTAACGCGA
TACGCGTTAGCGTGCGGTATAAAGCACCCGCAAGTTTTACTTTACTAA

Structures

Antitoxin

• AlphaFold DB: S1FJN6