Detailed information of TA system TA176332

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 3597418..3597643
• Replicon: chromosome
• Accession: NZ_CP062763
• Organism: Escherichia coli O157:H7 strain Z885

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: -
• Locus tag: ILQ36_RS18490
• Protein ID: WP_000813263.1
• Coordinates: 3597418..3597573 (-)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	3597585..3597643 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ILQ36_RS18455	3592872..3593585	-	714	WP_000261909.1	helix-turn-helix domain-containing protein	-
ILQ36_RS18460	3593723..3593919	-	197	Protein_3613	TrmB family transcriptional regulator	-
ILQ36_RS18465	3594206..3595024	-	819	WP_000265265.1	CPBP family intramembrane metalloprotease	-
ILQ36_RS18470	3595176..3595547	-	372	WP_000090264.1	antitermination protein	-
ILQ36_RS18475	3595537..3595908	-	372	WP_001217436.1	RusA family crossover junction endodeoxyribonuclease	-
ILQ36_RS18480	3595921..3596970	-	1050	WP_001265172.1	DUF968 domain-containing protein	-
ILQ36_RS18485	3596972..3597250	-	279	WP_001341388.1	hypothetical protein	-
ILQ36_RS18490	3597418..3597573	-	156	WP_000813263.1	type I toxin-antitoxin system toxin HokD	Toxin
-	3597585..3597643	+	59	-	-	Antitoxin
ILQ36_RS18495	3598178..3598951	+	774	WP_000160654.1	alpha/beta hydrolase	-
ILQ36_RS18500	3599303..3599716	-	414	WP_001151233.1	DUF977 family protein	-
ILQ36_RS18505	3599732..3600502	-	771	WP_000450992.1	DUF1627 domain-containing protein	-
ILQ36_RS18510	3600524..3601270	-	747	WP_000788751.1	ATP-binding protein	-
ILQ36_RS18515	3601277..3602368	-	1092	WP_001205823.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5722.86 Da
• Isoelectric Point: 6.1531

>T176332 WP_000813263.1 NZ_CP062763:c3597573-3597418 [Escherichia coli O157:H7]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRVRTGQTEVAVFTAYEPEE

Nucleotide

• Length: 156 bp

>T176332 NZ_CP082780:c254253-253495 [Bacillus aquiflavi]
ATGATTTTATTTTTTAAACTGTTTGTTTGGGCCTGCGTCGGCGGAATTGTTATTTATGTGTTTTCTTTTTGGAAATGGGA
AAAAAGTATGCGAAAAAAAATTTATGCGATTCGAAAAACATGGTATGTTATTTATCTTTTTGGAGCTGTAATTTTTTGGA
CTTATGAGCCGACCTCAATTTTTACTCATTGGCAAAACTATTTAATTGTTGCAGTAATTGTAGTGTTAGTAGATGCGTTT
GTATTTTTAAGCACTTATATTAAAAAGTTAGGCCATCACGAATTAGCCACAGATACGAAGGAGCTTCTTGAAGAAAACAG
GGACATGATGAAGTCGCAACAGAATAAGCTTAAAACATTTCACTATTTATTGAAAAATGAACCGATTAACATTTATTACG
GAGACATCGAAGATTATATAACTGGCATACAAGAACTGTTAAATGCTTTCGGCGAAAAAATCGACATGATTGCAACGATT
CATTCATACGCTACGCCTAAAGAAAAGTCATTTGTAATGAGAGAAATCACGAACCGCTCAGCAGCACAATCAGTGCTAGA
GAGACAAGAAGTATTTTACAGCGATCAAGAAAAGCTCGCCTTAATCCCGATGATTTTACAAGAACACTCTTATGTTTTAA
AACTCAAATCAGCAGATTTTGTCACAGAATTTGATTACCTTCTATTTACCTCGCTAATCTCCATGTATGACTTAATATAT
GCACCGTTTGAATATGAAGAGGATGGTGATGAAGTGTGA

Antitoxin

Nucleotide

• Length: 59 bp

>AT176332 NZ_CP062763:3597585-3597643 [Escherichia coli O157:H7]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTAGATTTGCCGTTCATAGATTGAGCCTCA

Structures

Antitoxin