Detailed information of TA system TA176299

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 4487913..4488607
• Replicon: chromosome
• Accession: NZ_CP062761
• Organism: Escherichia coli O157:H7 strain Z887

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: C3TNX7
• Locus tag: ILQ37_RS22660
• Protein ID: WP_001263495.1
• Coordinates: 4487913..4488311 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: ILQ37_RS22665
• Protein ID: WP_000554757.1
• Coordinates: 4488314..4488607 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
-	4483494..4483574	-	81	NuclAT_11	-	-
-	4483494..4483574	-	81	NuclAT_11	-	-
-	4483494..4483574	-	81	NuclAT_11	-	-
-	4483494..4483574	-	81	NuclAT_11	-	-
ILQ37_RS22635	4484170..4484628	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
ILQ37_RS22640	4484889..4486346	+	1458	WP_001293009.1	cytosol nonspecific dipeptidase	-
ILQ37_RS22645	4486403..4486930	-	528	Protein_4432	peptide chain release factor H	-
ILQ37_RS22650	4486929..4487132	-	204	Protein_4433	RNA ligase RtcB family protein	-
ILQ37_RS22655	4487451..4487903	-	453	WP_001059871.1	GNAT family N-acetyltransferase	-
ILQ37_RS22660	4487913..4488311	-	399	WP_001263495.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
ILQ37_RS22665	4488314..4488607	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
ILQ37_RS22670	4488659..4489714	-	1056	WP_001226186.1	DNA polymerase IV	-
ILQ37_RS22675	4489785..4490570	-	786	WP_000207556.1	putative lateral flagellar export/assembly protein LafU	-
ILQ37_RS22680	4490542..4492254	+	1713	Protein_4439	flagellar biosynthesis protein FlhA	-
ILQ37_RS22685	4492399..4492896	-	498	Protein_4440	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15473.86 Da
• Isoelectric Point: 8.0949

>T176299 WP_001263495.1 NZ_CP062761:c4488311-4487913 [Escherichia coli O157:H7]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDESHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T176299 NZ_CP082776:2918269-2918643 [Escherichia coli O4:H5]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTACGGCCTCACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT176299 WP_000554757.1 NZ_CP062761:c4488607-4488314 [Escherichia coli O157:H7]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT176299 NZ_CP082776:2917812-2918180 [Escherichia coli O4:H5]
GTGTCAGACACACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
CCAGCGATGCAGATGCGTACCCCCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCAATCAGCATACCGTCACGCTGTATGCGAAGGGGCTGGCCTGTGAAGCCGATACCCTCAGCAGTTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Structures

Antitoxin

• AlphaFold DB: S1FJN6