Detailed information of TA system TA176294

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 3562552..3562777
• Replicon: chromosome
• Accession: NZ_CP062761
• Organism: Escherichia coli O157:H7 strain Z887

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: -
• Locus tag: ILQ37_RS18250
• Protein ID: WP_000813263.1
• Coordinates: 3562552..3562707 (-)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	3562719..3562777 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ILQ37_RS18215	3558006..3558719	-	714	WP_000261909.1	helix-turn-helix domain-containing protein	-
ILQ37_RS18220	3558857..3559053	-	197	Protein_3565	TrmB family transcriptional regulator	-
ILQ37_RS18225	3559340..3560158	-	819	WP_000265265.1	CPBP family intramembrane metalloprotease	-
ILQ37_RS18230	3560310..3560681	-	372	WP_000090264.1	antitermination protein	-
ILQ37_RS18235	3560671..3561042	-	372	WP_001217436.1	RusA family crossover junction endodeoxyribonuclease	-
ILQ37_RS18240	3561055..3562104	-	1050	WP_001265172.1	DUF968 domain-containing protein	-
ILQ37_RS18245	3562106..3562384	-	279	WP_001341388.1	hypothetical protein	-
ILQ37_RS18250	3562552..3562707	-	156	WP_000813263.1	type I toxin-antitoxin system toxin HokD	Toxin
-	3562719..3562777	+	59	-	-	Antitoxin
ILQ37_RS18255	3563312..3564085	+	774	WP_000160654.1	alpha/beta hydrolase	-
ILQ37_RS18260	3564437..3564850	-	414	WP_001151233.1	DUF977 family protein	-
ILQ37_RS18265	3564866..3565636	-	771	WP_000450992.1	DUF1627 domain-containing protein	-
ILQ37_RS18270	3565658..3566404	-	747	WP_000788751.1	ATP-binding protein	-
ILQ37_RS18275	3566411..3567502	-	1092	WP_001205823.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5722.86 Da
• Isoelectric Point: 6.1531

>T176294 WP_000813263.1 NZ_CP062761:c3562707-3562552 [Escherichia coli O157:H7]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRVRTGQTEVAVFTAYEPEE

Nucleotide

• Length: 156 bp

>T176294 NZ_CP082776:c1281054-1280836 [Escherichia coli O4:H5]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT176294 NZ_CP062761:3562719-3562777 [Escherichia coli O157:H7]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTAGATTTGCCGTTCATAGATTGAGCCTCA

Structures

Antitoxin