Detailed information of TA system TA176290

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 2884335..2884560
• Replicon: chromosome
• Accession: NZ_CP062761
• Organism: Escherichia coli O157:H7 strain Z887

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: A0A1Q4PF16
• Locus tag: ILQ37_RS14790
• Protein ID: WP_000813258.1
• Coordinates: 2884405..2884560 (+)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	2884335..2884393 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ILQ37_RS14740	2879400..2879642	+	243	WP_000747948.1	hypothetical protein	-
ILQ37_RS14745	2879626..2880051	+	426	WP_000693878.1	Rha family transcriptional regulator	-
ILQ37_RS14750	2880120..2881163	+	1044	WP_001262402.1	hypothetical protein	-
ILQ37_RS14755	2881156..2881617	+	462	WP_000139447.1	replication protein	-
ILQ37_RS14760	2881651..2882367	+	717	WP_000450627.1	DUF1627 domain-containing protein	-
ILQ37_RS14765	2882400..2882681	+	282	WP_000603384.1	DNA-binding protein	-
ILQ37_RS14770	2882678..2882905	+	228	WP_000699809.1	hypothetical protein	-
ILQ37_RS14775	2882898..2883209	+	312	WP_001289673.1	hypothetical protein	-
ILQ37_RS14780	2883337..2883555	+	219	WP_000683609.1	DUF4014 family protein	-
ILQ37_RS14785	2883557..2884114	+	558	WP_000104474.1	DUF551 domain-containing protein	-
-	2884335..2884393	-	59	-	-	Antitoxin
ILQ37_RS14790	2884405..2884560	+	156	WP_000813258.1	type I toxin-antitoxin system Hok family toxin	Toxin
ILQ37_RS14795	2884680..2885024	+	345	WP_000756596.1	YgiW/YdeI family stress tolerance OB fold protein	-
ILQ37_RS14800	2885146..2885418	+	273	WP_000191872.1	hypothetical protein	-
ILQ37_RS14805	2885420..2886469	+	1050	WP_001265229.1	DUF968 domain-containing protein	-
ILQ37_RS14810	2886482..2886787	+	306	WP_001217444.1	RusA family crossover junction endodeoxyribonuclease	-
ILQ37_RS14815	2886850..2887404	+	555	WP_000640035.1	DUF1133 family protein	-
ILQ37_RS14820	2887629..2887826	+	198	WP_000917763.1	hypothetical protein	-
ILQ37_RS14825	2887962..2888675	+	714	WP_000301797.1	helix-turn-helix domain-containing protein	-
ILQ37_RS14840	2889126..2889557	+	432	WP_001302123.1	tellurite resistance protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	paa / nleG7'	2773515..2928474	154959

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5767.95 Da
• Isoelectric Point: 7.7942

>T176290 WP_000813258.1 NZ_CP062761:2884405-2884560 [Escherichia coli O157:H7]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFVDYESEK

Nucleotide

• Length: 156 bp

>T176290 NZ_CP082776:476188-476361 [Escherichia coli O4:H5]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTTCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Nucleotide

• Length: 59 bp

>AT176290 NZ_CP062761:c2884393-2884335 [Escherichia coli O157:H7]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACATGTGTTCAGCATGGATTGAGCCTCA

Structures

Toxin

• AlphaFold DB: A0A1Q4PF16

Antitoxin