176261

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 4461604..4462298 Replicon chromosome
Accession NZ_CP062758
Organism Escherichia coli O157:H7 strain Z892

Toxin (Protein)


Gene name yafO Uniprot ID C3TNX7
Locus tag ILQ38_RS22485 Protein ID WP_001263495.1
Coordinates 4461604..4462002 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1FJN6
Locus tag ILQ38_RS22490 Protein ID WP_000554757.1
Coordinates 4462005..4462298 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
- 4457191..4457271 - 81 NuclAT_11 - -
- 4457191..4457271 - 81 NuclAT_11 - -
- 4457191..4457271 - 81 NuclAT_11 - -
- 4457191..4457271 - 81 NuclAT_11 - -
ILQ38_RS22460 4457867..4458325 - 459 WP_001291990.1 xanthine phosphoribosyltransferase -
ILQ38_RS22465 4458586..4460043 + 1458 WP_001293009.1 cytosol nonspecific dipeptidase -
ILQ38_RS22470 4460100..4460621 - 522 Protein_4398 peptide chain release factor H -
ILQ38_RS22475 4460620..4460823 - 204 Protein_4399 RNA ligase RtcB family protein -
ILQ38_RS22480 4461142..4461594 - 453 WP_001059871.1 GNAT family N-acetyltransferase -
ILQ38_RS22485 4461604..4462002 - 399 WP_001263495.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
ILQ38_RS22490 4462005..4462298 - 294 WP_000554757.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
ILQ38_RS22495 4462350..4463405 - 1056 WP_001226186.1 DNA polymerase IV -
ILQ38_RS22500 4463476..4464261 - 786 WP_000207556.1 putative lateral flagellar export/assembly protein LafU -
ILQ38_RS22505 4464233..4465945 + 1713 Protein_4405 flagellar biosynthesis protein FlhA -
ILQ38_RS22510 4466090..4466587 - 498 Protein_4406 REP-associated tyrosine transposase RayT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15473.86 Da        Isoelectric Point: 8.0949

>T176261 WP_001263495.1 NZ_CP062758:c4462002-4461604 [Escherichia coli O157:H7]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDESHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T176261 NZ_CP082774:c2098668-2098561 [Escherichia coli O16:H6]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCAGCACCGATCCTTGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.81 Da        Isoelectric Point: 5.9050

>AT176261 WP_000554757.1 NZ_CP062758:c4462298-4462005 [Escherichia coli O157:H7]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT176261 NZ_CP082774:2098716-2098782 [Escherichia coli O16:H6]
GTTCTGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTATACCTCTCAACGTGCGGGGGTTTTCTC

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
AlphaFold DB S1FJN6

References