Detailed information of TA system TA176256

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 3575007..3575232
• Replicon: chromosome
• Accession: NZ_CP062758
• Organism: Escherichia coli O157:H7 strain Z892

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: -
• Locus tag: ILQ38_RS18370
• Protein ID: WP_000813263.1
• Coordinates: 3575007..3575162 (-)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	3575174..3575232 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ILQ38_RS18335	3570461..3571174	-	714	WP_000261909.1	helix-turn-helix domain-containing protein	-
ILQ38_RS18340	3571312..3571508	-	197	Protein_3589	TrmB family transcriptional regulator	-
ILQ38_RS18345	3571795..3572613	-	819	WP_000265265.1	CPBP family intramembrane metalloprotease	-
ILQ38_RS18350	3572765..3573136	-	372	WP_000090264.1	antitermination protein	-
ILQ38_RS18355	3573126..3573497	-	372	WP_001217436.1	RusA family crossover junction endodeoxyribonuclease	-
ILQ38_RS18360	3573510..3574559	-	1050	WP_001265172.1	DUF968 domain-containing protein	-
ILQ38_RS18365	3574561..3574839	-	279	WP_001341388.1	hypothetical protein	-
ILQ38_RS18370	3575007..3575162	-	156	WP_000813263.1	type I toxin-antitoxin system toxin HokD	Toxin
-	3575174..3575232	+	59	-	-	Antitoxin
ILQ38_RS18375	3575767..3576540	+	774	WP_000160654.1	alpha/beta hydrolase	-
ILQ38_RS18380	3576892..3577305	-	414	WP_001151233.1	DUF977 family protein	-
ILQ38_RS18385	3577321..3578091	-	771	WP_000450992.1	DUF1627 domain-containing protein	-
ILQ38_RS18390	3578113..3578859	-	747	WP_000788751.1	ATP-binding protein	-
ILQ38_RS18395	3578866..3579957	-	1092	WP_001205823.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5722.86 Da
• Isoelectric Point: 6.1531

>T176256 WP_000813263.1 NZ_CP062758:c3575162-3575007 [Escherichia coli O157:H7]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRVRTGQTEVAVFTAYEPEE

Nucleotide

• Length: 156 bp

>T176256 NZ_CP082774:862786-863073 [Escherichia coli O16:H6]
ATGCCAATGGAGTTTGAATGGGATGAAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTATTGTTTGATGATCCACAACATTTGTCACAGCAAGAACGCATAGAAAACGGTGAGTATCGCTGGCAGACAATCGGCC
TGGTTTACGGCATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAATCGCTATGAGCATGGTTAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT176256 NZ_CP062758:3575174-3575232 [Escherichia coli O157:H7]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTAGATTTGCCGTTCATAGATTGAGCCTCA

Structures

Antitoxin