Detailed information of TA system TA176253

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 2997109..2997334
• Replicon: chromosome
• Accession: NZ_CP062758
• Organism: Escherichia coli O157:H7 strain Z892

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: A0A1Q4PF16
• Locus tag: ILQ38_RS15110
• Protein ID: WP_000813258.1
• Coordinates: 2997109..2997264 (-)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	2997276..2997334 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ILQ38_RS15060	2992112..2992543	-	432	WP_001302123.1	tellurite resistance protein	-
ILQ38_RS15075	2992994..2993707	-	714	WP_000301797.1	helix-turn-helix domain-containing protein	-
ILQ38_RS15080	2993843..2994040	-	198	WP_000917763.1	hypothetical protein	-
ILQ38_RS15085	2994265..2994819	-	555	WP_000640035.1	DUF1133 family protein	-
ILQ38_RS15090	2994882..2995187	-	306	WP_001217444.1	RusA family crossover junction endodeoxyribonuclease	-
ILQ38_RS15095	2995200..2996249	-	1050	WP_001265229.1	DUF968 domain-containing protein	-
ILQ38_RS15100	2996251..2996523	-	273	WP_000191872.1	hypothetical protein	-
ILQ38_RS15105	2996645..2996989	-	345	WP_000756596.1	YgiW/YdeI family stress tolerance OB fold protein	-
ILQ38_RS15110	2997109..2997264	-	156	WP_000813258.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	2997276..2997334	+	59	-	-	Antitoxin
ILQ38_RS15115	2997555..2998112	-	558	WP_000104474.1	DUF551 domain-containing protein	-
ILQ38_RS15120	2998114..2998332	-	219	WP_000683609.1	DUF4014 family protein	-
ILQ38_RS15125	2998460..2998771	-	312	WP_001289673.1	hypothetical protein	-
ILQ38_RS15130	2998764..2998991	-	228	WP_000699809.1	hypothetical protein	-
ILQ38_RS15135	2998988..2999269	-	282	WP_000603384.1	DNA-binding protein	-
ILQ38_RS15140	2999302..3000018	-	717	WP_000450627.1	DUF1627 domain-containing protein	-
ILQ38_RS15145	3000052..3000513	-	462	WP_000139447.1	replication protein	-
ILQ38_RS15150	3000506..3001549	-	1044	WP_001262402.1	hypothetical protein	-
ILQ38_RS15155	3001618..3002043	-	426	WP_000693878.1	Rha family transcriptional regulator	-
ILQ38_RS15160	3002027..3002269	-	243	WP_000747948.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	nleC / nleG7' / paa	2914193..3061859	147666

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5767.95 Da
• Isoelectric Point: 7.7942

>T176253 WP_000813258.1 NZ_CP062758:c2997264-2997109 [Escherichia coli O157:H7]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFVDYESEK

Nucleotide

• Length: 156 bp

>T176253 NZ_CP082774:c491934-491557 [Escherichia coli O16:H6]
ATGAAAACATTACCCGATACACACGTACGGGAGGCATCGAGCTGCCCGTCTCCCGTCACCATCTGGCAGACTCTGCTCTC
CCGACTACTGGGCCAGCATTACGGCCTTACCCTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCACTGGTGCGTACCGACCAGCCGGAATTC
AGTGCCTGTACCCGTTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCACGCCGGGCAACCGGCCTGATGACCCGCGA
CAACTACAGAACGGTAAATGACATTACCCTGGGTAAGCATCCGGAGGCGAAACAATGA

Antitoxin

Nucleotide

• Length: 59 bp

>AT176253 NZ_CP062758:2997276-2997334 [Escherichia coli O157:H7]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACATGTGTTCAGCATGGATTGAGCCTCA

Structures

Toxin

• AlphaFold DB: A0A1Q4PF16

Antitoxin