Detailed information of TA system TA176239

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 702015..702814
• Replicon: chromosome
• Accession: NZ_CP062758
• Organism: Escherichia coli O157:H7 strain Z892

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: V0SSH7
• Locus tag: ILQ38_RS03425
• Protein ID: WP_000347273.1
• Coordinates: 702015..702479 (-)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: C3ST72
• Locus tag: ILQ38_RS03430
• Protein ID: WP_001302819.1
• Coordinates: 702479..702814 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ILQ38_RS03395	697016..697450	-	435	WP_000948824.1	PTS sugar transporter subunit IIA	-
ILQ38_RS03400	697468..698346	-	879	WP_001295548.1	PTS system mannose/fructose/sorbose family transporter subunit IID	-
ILQ38_RS03405	698336..699115	-	780	WP_000406214.1	PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC	-
ILQ38_RS03410	699126..699599	-	474	WP_001301475.1	PTS N-acetylgalactosamine transporter subunit IIB	-
ILQ38_RS03415	699622..700902	-	1281	WP_000681927.1	tagatose-bisphosphate aldolase subunit KbaZ	-
ILQ38_RS03420	701151..701960	+	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
ILQ38_RS03425	702015..702479	-	465	WP_000347273.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
ILQ38_RS03430	702479..702814	-	336	WP_001302819.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
ILQ38_RS03435	702963..704534	-	1572	WP_001273776.1	galactarate dehydratase	-
ILQ38_RS03440	704909..706243	+	1335	WP_000599636.1	galactarate/glucarate/glycerate transporter GarP	-
ILQ38_RS03445	706259..707029	+	771	WP_001058227.1	2-dehydro-3-deoxyglucarate aldolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17836.25 Da
• Isoelectric Point: 9.6924

>T176239 WP_000347273.1 NZ_CP062758:c702479-702015 [Escherichia coli O157:H7]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Nucleotide

• Length: 465 bp

>T176239 NZ_CP082773:c2435861-2435583 [Escherichia coli O99:H6]
ATGATAATGAATTTCAGACACAAGGGATTGCGTGACTTGTTTCTTCTCGGCAAAACCTCCGGCGTTATACCGACGCAAGT
CAAACGATTACGCCACCGACTCGCTGTGATTGATGCAGCATGCTGTCTTGCTGATATCGATATGCCCGGTTACCGACTAC
ATCCGTTAAGCGGCGATCGCGATGGAATTTGGGCGATATCTGTCTCGGGCAACTGGCGAATCACATTTGAATTCGTCAAT
GGCGATGCATACATACTGGATTACGAGGACTATCACTGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12388.97 Da
• Isoelectric Point: 4.8616

>AT176239 WP_001302819.1 NZ_CP062758:c702814-702479 [Escherichia coli O157:H7]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDTDIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT176239 NZ_CP082773:c2435583-2435299 [Escherichia coli O99:H6]
ATGAAAATGGCCAATCATCCCCGCCCGGGGGACATTATTCAGGAATCACTGGACGAACTTAATGTCAGCCTGCGCGAGTT
TGCCAGAGCAATGGAAATTGCGCCCTCAACGGCAAGCCGATTGCTGACCGGAAAAGCAGCTTTGACGCCAGAAATGGCAA
TAAAACTTTCCGTGGTGATCGGCAGTTCGCCGCAAATGTGGCTGAATCTGCAAAATGCCTGGAGTCTGGCAGAGGCAGAA
AAAACAGTGGATGTGTCGCGACTGCGCCGTCTGGTTACGCAATAA

Structures

Toxin

• AlphaFold DB: V0SSH7