Detailed information of TA system TA176220

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 3585862..3586087
• Replicon: chromosome
• Accession: NZ_CP062755
• Organism: Escherichia coli O157:H7 strain Z903

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: -
• Locus tag: ILQ39_RS18425
• Protein ID: WP_000813263.1
• Coordinates: 3585862..3586017 (-)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	3586029..3586087 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ILQ39_RS18390	3581316..3582029	-	714	WP_000261909.1	helix-turn-helix domain-containing protein	-
ILQ39_RS18395	3582167..3582363	-	197	Protein_3600	TrmB family transcriptional regulator	-
ILQ39_RS18400	3582650..3583468	-	819	WP_000265265.1	CPBP family intramembrane metalloprotease	-
ILQ39_RS18405	3583620..3583991	-	372	WP_000090264.1	antitermination protein	-
ILQ39_RS18410	3583981..3584352	-	372	WP_001217436.1	RusA family crossover junction endodeoxyribonuclease	-
ILQ39_RS18415	3584365..3585414	-	1050	WP_001265172.1	DUF968 domain-containing protein	-
ILQ39_RS18420	3585416..3585694	-	279	WP_001341388.1	hypothetical protein	-
ILQ39_RS18425	3585862..3586017	-	156	WP_000813263.1	type I toxin-antitoxin system toxin HokD	Toxin
-	3586029..3586087	+	59	-	-	Antitoxin
ILQ39_RS18430	3586622..3587395	+	774	WP_000160654.1	alpha/beta hydrolase	-
ILQ39_RS18435	3587747..3588160	-	414	WP_001151233.1	DUF977 family protein	-
ILQ39_RS18440	3588176..3588946	-	771	WP_000450992.1	DUF1627 domain-containing protein	-
ILQ39_RS18445	3588968..3589714	-	747	WP_000788751.1	ATP-binding protein	-
ILQ39_RS18450	3589721..3590812	-	1092	WP_001205823.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5722.86 Da
• Isoelectric Point: 6.1531

>T176220 WP_000813263.1 NZ_CP062755:c3586017-3585862 [Escherichia coli O157:H7]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRVRTGQTEVAVFTAYEPEE

Nucleotide

• Length: 156 bp

>T176220 NZ_CP082772:3190951-3191325 [Escherichia coli O2:H1]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTACGGCCTCACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin

Nucleotide

• Length: 59 bp

>AT176220 NZ_CP062755:3586029-3586087 [Escherichia coli O157:H7]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTAGATTTGCCGTTCATAGATTGAGCCTCA

Structures

Antitoxin