Detailed information of TA system TA176190

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): DinJ-YafQ (relBE)/YafQ-RelB
• Location: 4465477..4466016
• Replicon: chromosome
• Accession: NZ_CP062752
• Organism: Escherichia coli O157:H7 strain Z910

Toxin (Protein)

• Gene name: yafQ
• Uniprot ID: -
• Locus tag: ILQ40_RS22510
• Protein ID: WP_001303998.1
• Coordinates: 4465756..4466016 (+)
• Length: 87 a.a.

Antitoxin (Protein)

• Gene name: dinJ
• Uniprot ID: C3TP07
• Locus tag: ILQ40_RS22505
• Protein ID: WP_000729705.1
• Coordinates: 4465477..4465737 (+)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ILQ40_RS22485	4461231..4462016	-	786	WP_000207556.1	putative lateral flagellar export/assembly protein LafU	-
ILQ40_RS22490	4461988..4463700	+	1713	Protein_4402	flagellar biosynthesis protein FlhA	-
ILQ40_RS22495	4463845..4464342	-	498	Protein_4403	REP-associated tyrosine transposase RayT	-
ILQ40_RS22500	4464518..4465291	-	774	WP_000543891.1	C40 family peptidase	-
ILQ40_RS22505	4465477..4465737	+	261	WP_000729705.1	type II toxin-antitoxin system antitoxin DinJ	Antitoxin
ILQ40_RS22510	4465756..4466016	+	261	WP_001303998.1	type II toxin-antitoxin system mRNA interferase toxin YafQ	Toxin
ILQ40_RS22515	4466172..4466912	+	741	WP_001225679.1	murein L,D-transpeptidase	-
ILQ40_RS22520	4466883..4467650	-	768	WP_001301698.1	class II glutamine amidotransferase	-
ILQ40_RS22525	4467755..4468333	-	579	WP_000284050.1	D-sedoheptulose 7-phosphate isomerase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (3-85)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-85)

Sequences

Toxin

Protein

• Length: 87 a.a.
• Molecular weight: 10026.71 Da
• Isoelectric Point: 10.3188

>T176190 WP_001303998.1 NZ_CP062752:4465756-4466016 [Escherichia coli O157:H7]
MNSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLRFERTGT
HAALFG

Nucleotide

• Length: 261 bp

>T176190 NZ_CP082771:473327-473500 [Escherichia coli LF82]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTTCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9377.74 Da
• Isoelectric Point: 5.0758

>AT176190 WP_000729705.1 NZ_CP062752:4465477-4465737 [Escherichia coli O157:H7]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGV

Nucleotide

• Length: 261 bp

>AT176190 NZ_CP082771:473003-473299 [Escherichia coli LF82]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAACTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAGCATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG