Detailed information of TA system TA176189

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 4459359..4460053
• Replicon: chromosome
• Accession: NZ_CP062752
• Organism: Escherichia coli O157:H7 strain Z910

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: C3TNX7
• Locus tag: ILQ40_RS22470
• Protein ID: WP_001263495.1
• Coordinates: 4459359..4459757 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: ILQ40_RS22475
• Protein ID: WP_000554757.1
• Coordinates: 4459760..4460053 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
-	4454946..4455026	-	81	NuclAT_11	-	-
-	4454946..4455026	-	81	NuclAT_11	-	-
-	4454946..4455026	-	81	NuclAT_11	-	-
-	4454946..4455026	-	81	NuclAT_11	-	-
ILQ40_RS22445	4455622..4456080	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
ILQ40_RS22450	4456341..4457798	+	1458	WP_001293009.1	cytosol nonspecific dipeptidase	-
ILQ40_RS22455	4457855..4458376	-	522	Protein_4395	peptide chain release factor H	-
ILQ40_RS22460	4458375..4458578	-	204	Protein_4396	RNA ligase RtcB family protein	-
ILQ40_RS22465	4458897..4459349	-	453	WP_001059871.1	GNAT family N-acetyltransferase	-
ILQ40_RS22470	4459359..4459757	-	399	WP_001263495.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
ILQ40_RS22475	4459760..4460053	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
ILQ40_RS22480	4460105..4461160	-	1056	WP_001226186.1	DNA polymerase IV	-
ILQ40_RS22485	4461231..4462016	-	786	WP_000207556.1	putative lateral flagellar export/assembly protein LafU	-
ILQ40_RS22490	4461988..4463700	+	1713	Protein_4402	flagellar biosynthesis protein FlhA	-
ILQ40_RS22495	4463845..4464342	-	498	Protein_4403	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15473.86 Da
• Isoelectric Point: 8.0949

>T176189 WP_001263495.1 NZ_CP062752:c4459757-4459359 [Escherichia coli O157:H7]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDESHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T176189 NZ_CP082771:174878-175189 [Escherichia coli LF82]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGAACGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTCTCAGGAG
GAAAGGGGAAATGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT176189 WP_000554757.1 NZ_CP062752:c4460053-4459760 [Escherichia coli O157:H7]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT176189 NZ_CP082771:175190-175480 [Escherichia coli LF82]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
ACGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Antitoxin

• AlphaFold DB: S1FJN6