Detailed information of TA system TA176184

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 3574066..3574291
• Replicon: chromosome
• Accession: NZ_CP062752
• Organism: Escherichia coli O157:H7 strain Z910

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: -
• Locus tag: ILQ40_RS18360
• Protein ID: WP_000813263.1
• Coordinates: 3574066..3574221 (-)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	3574233..3574291 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ILQ40_RS18325	3569520..3570233	-	714	WP_000261909.1	helix-turn-helix domain-containing protein	-
ILQ40_RS18330	3570371..3570567	-	197	Protein_3587	TrmB family transcriptional regulator	-
ILQ40_RS18335	3570854..3571672	-	819	WP_000265265.1	CPBP family intramembrane metalloprotease	-
ILQ40_RS18340	3571824..3572195	-	372	WP_000090264.1	antitermination protein	-
ILQ40_RS18345	3572185..3572556	-	372	WP_001217436.1	RusA family crossover junction endodeoxyribonuclease	-
ILQ40_RS18350	3572569..3573618	-	1050	WP_001265172.1	DUF968 domain-containing protein	-
ILQ40_RS18355	3573620..3573898	-	279	WP_001341388.1	hypothetical protein	-
ILQ40_RS18360	3574066..3574221	-	156	WP_000813263.1	type I toxin-antitoxin system toxin HokD	Toxin
-	3574233..3574291	+	59	-	-	Antitoxin
ILQ40_RS18365	3574826..3575599	+	774	WP_000160654.1	alpha/beta hydrolase	-
ILQ40_RS18370	3575951..3576364	-	414	WP_001151233.1	DUF977 family protein	-
ILQ40_RS18375	3576380..3577150	-	771	WP_000450992.1	DUF1627 domain-containing protein	-
ILQ40_RS18380	3577172..3577918	-	747	WP_000788751.1	ATP-binding protein	-
ILQ40_RS18385	3577925..3579016	-	1092	WP_001205823.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5722.86 Da
• Isoelectric Point: 6.1531

>T176184 WP_000813263.1 NZ_CP062752:c3574221-3574066 [Escherichia coli O157:H7]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRVRTGQTEVAVFTAYEPEE

Nucleotide

• Length: 156 bp

>T176184 NZ_CP082766:c85763-85293 [Klebsiella pneumoniae]
GTGATCCTCTACAGGCTGACGAAAACAAAGTATCTTTCTACGGCCTGGACCGGATATGGTGCGAAAGAGGCTGGTGGGCG
TTGGAATAGCGTGGGGGTGTCAATGGTATATGTCTCCGAAACGGCATCACTAACGATGCTGGAGACGCTGGTACACCTGC
ATGCGGCACAGATAATGGACTCTTTCACGCTACTGAGTATCGATGTGCCTGATGAACTGATCCAAAGCGCAAACATGGAT
GAATTACCGGGCAACTGGGCTGATGAGGATGCGCCTCAGGAACTGGCTGATTACGGAGATGCCTGGTCATTTACCAGGAG
CTCGGTTGCACTGCGTGTTCCCTCAGCCTTATCGCCGGTAGAGTTCAACTATCTGTTAAATCCTGAGCACCCTGAGTTTT
ACGGGATCGTGCAGAAGGCTCAACAGATACCGTTCCGGTTTGATAGTCGTCTTAAACCTGACAGGAAGTGA

Antitoxin

Nucleotide

• Length: 59 bp

>AT176184 NZ_CP062752:3574233-3574291 [Escherichia coli O157:H7]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTAGATTTGCCGTTCATAGATTGAGCCTCA

Structures

Antitoxin