Detailed information of TA system TA176179

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 2648459..2648684
• Replicon: chromosome
• Accession: NZ_CP062752
• Organism: Escherichia coli O157:H7 strain Z910

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: A0A1Q4PF16
• Locus tag: ILQ40_RS13445
• Protein ID: WP_000813258.1
• Coordinates: 2648529..2648684 (+)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	2648459..2648517 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ILQ40_RS13395	2643524..2643766	+	243	WP_000747948.1	hypothetical protein	-
ILQ40_RS13400	2643750..2644175	+	426	WP_000693878.1	Rha family transcriptional regulator	-
ILQ40_RS13405	2644244..2645287	+	1044	WP_001262402.1	hypothetical protein	-
ILQ40_RS13410	2645280..2645741	+	462	WP_000139447.1	replication protein	-
ILQ40_RS13415	2645775..2646491	+	717	WP_000450627.1	DUF1627 domain-containing protein	-
ILQ40_RS13420	2646524..2646805	+	282	WP_000603384.1	DNA-binding protein	-
ILQ40_RS13425	2646802..2647029	+	228	WP_000699809.1	hypothetical protein	-
ILQ40_RS13430	2647022..2647333	+	312	WP_001289673.1	hypothetical protein	-
ILQ40_RS13435	2647461..2647679	+	219	WP_000683609.1	DUF4014 family protein	-
ILQ40_RS13440	2647681..2648238	+	558	WP_000104474.1	DUF551 domain-containing protein	-
-	2648459..2648517	-	59	-	-	Antitoxin
ILQ40_RS13445	2648529..2648684	+	156	WP_000813258.1	type I toxin-antitoxin system Hok family toxin	Toxin
ILQ40_RS13450	2648804..2649148	+	345	WP_000756596.1	YgiW/YdeI family stress tolerance OB fold protein	-
ILQ40_RS13455	2649270..2649542	+	273	WP_000191872.1	hypothetical protein	-
ILQ40_RS13460	2649544..2650593	+	1050	WP_001265229.1	DUF968 domain-containing protein	-
ILQ40_RS13465	2650606..2650911	+	306	WP_001217444.1	RusA family crossover junction endodeoxyribonuclease	-
ILQ40_RS13470	2650974..2651528	+	555	WP_000640035.1	DUF1133 family protein	-
ILQ40_RS13475	2651753..2651950	+	198	WP_000917763.1	hypothetical protein	-
ILQ40_RS13480	2652086..2652799	+	714	WP_000301797.1	helix-turn-helix domain-containing protein	-
ILQ40_RS13495	2653250..2653681	+	432	WP_001302123.1	tellurite resistance protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	paa / nleG7' / nleC	2579065..2738589	159524

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5767.95 Da
• Isoelectric Point: 7.7942

>T176179 WP_000813258.1 NZ_CP062752:2648529-2648684 [Escherichia coli O157:H7]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFVDYESEK

Nucleotide

• Length: 156 bp

>T176179 NZ_CP082765:c4921079-4920795 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGGCCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT176179 NZ_CP062752:c2648517-2648459 [Escherichia coli O157:H7]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACATGTGTTCAGCATGGATTGAGCCTCA

Structures

Toxin

• AlphaFold DB: A0A1Q4PF16

Antitoxin