Detailed information of TA system TA176153

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 4471118..4471812
• Replicon: chromosome
• Accession: NZ_CP062749
• Organism: Escherichia coli O157:H7 strain Z1486

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: C3TNX7
• Locus tag: ILQ41_RS22535
• Protein ID: WP_001263495.1
• Coordinates: 4471118..4471516 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: ILQ41_RS22540
• Protein ID: WP_000554757.1
• Coordinates: 4471519..4471812 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
-	4466705..4466785	-	81	NuclAT_11	-	-
-	4466705..4466785	-	81	NuclAT_11	-	-
-	4466705..4466785	-	81	NuclAT_11	-	-
-	4466705..4466785	-	81	NuclAT_11	-	-
ILQ41_RS22510	4467381..4467839	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
ILQ41_RS22515	4468100..4469557	+	1458	WP_001293009.1	cytosol nonspecific dipeptidase	-
ILQ41_RS22520	4469614..4470135	-	522	Protein_4408	peptide chain release factor H	-
ILQ41_RS22525	4470134..4470337	-	204	Protein_4409	RNA ligase RtcB family protein	-
ILQ41_RS22530	4470656..4471108	-	453	WP_001059871.1	GNAT family N-acetyltransferase	-
ILQ41_RS22535	4471118..4471516	-	399	WP_001263495.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
ILQ41_RS22540	4471519..4471812	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
ILQ41_RS22545	4471864..4472919	-	1056	WP_001226186.1	DNA polymerase IV	-
ILQ41_RS22550	4472990..4473775	-	786	WP_000207556.1	putative lateral flagellar export/assembly protein LafU	-
ILQ41_RS22555	4473747..4475459	+	1713	Protein_4415	flagellar biosynthesis protein FlhA	-
ILQ41_RS22560	4475604..4476101	-	498	Protein_4416	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15473.86 Da
• Isoelectric Point: 8.0949

>T176153 WP_001263495.1 NZ_CP062749:c4471516-4471118 [Escherichia coli O157:H7]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDESHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T176153 NZ_CP082759:328118-328486 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCAGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACTCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCGGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCGGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGCTTGCGCGGATGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT176153 WP_000554757.1 NZ_CP062749:c4471812-4471519 [Escherichia coli O157:H7]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT176153 NZ_CP082759:327900-328121 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGACGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Structures

Antitoxin

• AlphaFold DB: S1FJN6