Detailed information of TA system TA176148

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 3583214..3583439
• Replicon: chromosome
• Accession: NZ_CP062749
• Organism: Escherichia coli O157:H7 strain Z1486

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: -
• Locus tag: ILQ41_RS18405
• Protein ID: WP_000813263.1
• Coordinates: 3583214..3583369 (-)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	3583381..3583439 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ILQ41_RS18370	3578668..3579381	-	714	WP_000261909.1	helix-turn-helix domain-containing protein	-
ILQ41_RS18375	3579519..3579715	-	197	Protein_3596	TrmB family transcriptional regulator	-
ILQ41_RS18380	3580002..3580820	-	819	WP_000265265.1	CPBP family intramembrane metalloprotease	-
ILQ41_RS18385	3580972..3581343	-	372	WP_000090264.1	antitermination protein	-
ILQ41_RS18390	3581333..3581704	-	372	WP_032283490.1	RusA family crossover junction endodeoxyribonuclease	-
ILQ41_RS18395	3581717..3582766	-	1050	WP_206338758.1	DUF968 domain-containing protein	-
ILQ41_RS18400	3582768..3583046	-	279	WP_001341388.1	hypothetical protein	-
ILQ41_RS18405	3583214..3583369	-	156	WP_000813263.1	type I toxin-antitoxin system toxin HokD	Toxin
-	3583381..3583439	+	59	-	-	Antitoxin
ILQ41_RS18410	3583974..3584747	+	774	WP_000160654.1	alpha/beta hydrolase	-
ILQ41_RS18415	3585099..3585512	-	414	WP_001151233.1	DUF977 family protein	-
ILQ41_RS18420	3585528..3586298	-	771	WP_000450992.1	DUF1627 domain-containing protein	-
ILQ41_RS18425	3586320..3587066	-	747	WP_000788751.1	ATP-binding protein	-
ILQ41_RS18430	3587073..3588153	-	1081	Protein_3607	DNA-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5722.86 Da
• Isoelectric Point: 6.1531

>T176148 WP_000813263.1 NZ_CP062749:c3583369-3583214 [Escherichia coli O157:H7]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRVRTGQTEVAVFTAYEPEE

Nucleotide

• Length: 156 bp

>T176148 NZ_CP082754:c41044-40733 [Klebsiella pneumoniae]
ATGCACGTAATTTCAAAAGAGCCTTTTGAGGAAGCGGCAAAGCGATATCCCAACGATTCGTTAGCCATTCGGGCGTTGTA
CCGCTTAGTGCGCGAGACGGACTTCTCTTCTCCAGCTGAGATGCTAACTCTGATACCGAGCCTGGATAATTTTAAGTACA
GGAATAAGTGGTGGGTTTTGGATGTAGGGGGCAACAACTTAAGGGTTATCGCCTATATCAATTTCGTAAATAAGCGCTTC
TACGTGAAGCACATCGCAACCCACGCTGATTACGACAAGTTGACCCGCTATTACAGGGAGAACAAAGAATGA

Antitoxin

Nucleotide

• Length: 59 bp

>AT176148 NZ_CP062749:3583381-3583439 [Escherichia coli O157:H7]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTAGATTTGCCGTTCATAGATTGAGCCTCA

Structures

Antitoxin