Detailed information of TA system TA176118

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): DinJ-YafQ (relBE)/YafQ-RelB
• Location: 4464064..4464603
• Replicon: chromosome
• Accession: NZ_CP062746
• Organism: Escherichia coli O157:H7 strain Z1504

Toxin (Protein)

• Gene name: yafQ
• Uniprot ID: -
• Locus tag: ILQ42_RS22500
• Protein ID: WP_001303998.1
• Coordinates: 4464343..4464603 (+)
• Length: 87 a.a.

Antitoxin (Protein)

• Gene name: dinJ
• Uniprot ID: C3TP07
• Locus tag: ILQ42_RS22495
• Protein ID: WP_000729705.1
• Coordinates: 4464064..4464324 (+)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ILQ42_RS22475	4459818..4460603	-	786	WP_000207556.1	putative lateral flagellar export/assembly protein LafU	-
ILQ42_RS22480	4460575..4462287	+	1713	Protein_4401	flagellar biosynthesis protein FlhA	-
ILQ42_RS22485	4462432..4462929	-	498	Protein_4402	REP-associated tyrosine transposase RayT	-
ILQ42_RS22490	4463105..4463878	-	774	WP_000543891.1	C40 family peptidase	-
ILQ42_RS22495	4464064..4464324	+	261	WP_000729705.1	type II toxin-antitoxin system antitoxin DinJ	Antitoxin
ILQ42_RS22500	4464343..4464603	+	261	WP_001303998.1	type II toxin-antitoxin system mRNA interferase toxin YafQ	Toxin
ILQ42_RS22505	4464759..4465499	+	741	WP_001225679.1	murein L,D-transpeptidase	-
ILQ42_RS22510	4465470..4466237	-	768	WP_001301698.1	class II glutamine amidotransferase	-
ILQ42_RS22515	4466342..4466920	-	579	WP_000284050.1	D-sedoheptulose 7-phosphate isomerase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (3-85)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-85)

Sequences

Toxin

Protein

• Length: 87 a.a.
• Molecular weight: 10026.71 Da
• Isoelectric Point: 10.3188

>T176118 WP_001303998.1 NZ_CP062746:4464343-4464603 [Escherichia coli O157:H7]
MNSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLRFERTGT
HAALFG

Nucleotide

• Length: 261 bp

>T176118 NZ_CP082357:c4262527-4262354 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9377.74 Da
• Isoelectric Point: 5.0758

>AT176118 WP_000729705.1 NZ_CP062746:4464064-4464324 [Escherichia coli O157:H7]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGV

Nucleotide

• Length: 261 bp

>AT176118 NZ_CP082357:c4262851-4262555 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GACTTTGACCTCAACATTATGACAGTTGATGATTATTTCCGTCAGTTTTACAAGTAG