Detailed information of TA system TA176117

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 4457946..4458640
• Replicon: chromosome
• Accession: NZ_CP062746
• Organism: Escherichia coli O157:H7 strain Z1504

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: C3TNX7
• Locus tag: ILQ42_RS22460
• Protein ID: WP_001263495.1
• Coordinates: 4457946..4458344 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: ILQ42_RS22465
• Protein ID: WP_000554757.1
• Coordinates: 4458347..4458640 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
-	4453533..4453613	-	81	NuclAT_11	-	-
-	4453533..4453613	-	81	NuclAT_11	-	-
-	4453533..4453613	-	81	NuclAT_11	-	-
-	4453533..4453613	-	81	NuclAT_11	-	-
ILQ42_RS22435	4454209..4454667	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
ILQ42_RS22440	4454928..4456385	+	1458	WP_001293009.1	cytosol nonspecific dipeptidase	-
ILQ42_RS22445	4456442..4456963	-	522	Protein_4394	peptide chain release factor H	-
ILQ42_RS22450	4456962..4457165	-	204	Protein_4395	RNA ligase RtcB family protein	-
ILQ42_RS22455	4457484..4457936	-	453	WP_001059871.1	GNAT family N-acetyltransferase	-
ILQ42_RS22460	4457946..4458344	-	399	WP_001263495.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
ILQ42_RS22465	4458347..4458640	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
ILQ42_RS22470	4458692..4459747	-	1056	WP_001226186.1	DNA polymerase IV	-
ILQ42_RS22475	4459818..4460603	-	786	WP_000207556.1	putative lateral flagellar export/assembly protein LafU	-
ILQ42_RS22480	4460575..4462287	+	1713	Protein_4401	flagellar biosynthesis protein FlhA	-
ILQ42_RS22485	4462432..4462929	-	498	Protein_4402	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15473.86 Da
• Isoelectric Point: 8.0949

>T176117 WP_001263495.1 NZ_CP062746:c4458344-4457946 [Escherichia coli O157:H7]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDESHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T176117 NZ_CP082357:c4163380-4163030 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGGTGAGGTGGTGGAAGAGGC
GTTATTACGTTTGCAGGCGGTGGTGGAATAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT176117 WP_000554757.1 NZ_CP062746:c4458640-4458347 [Escherichia coli O157:H7]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT176117 NZ_CP082357:c4163625-4163374 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTTCAGGTATGGTCATTCCTAATGTTGTGATGAAAGAACTTAACTTACG
GCCGGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCACGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Structures

Antitoxin

• AlphaFold DB: S1FJN6