Detailed information of TA system TA176112

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 3571339..3571564
• Replicon: chromosome
• Accession: NZ_CP062746
• Organism: Escherichia coli O157:H7 strain Z1504

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: -
• Locus tag: ILQ42_RS18345
• Protein ID: WP_000813263.1
• Coordinates: 3571339..3571494 (-)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	3571506..3571564 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ILQ42_RS18310	3566793..3567506	-	714	WP_000261909.1	helix-turn-helix domain-containing protein	-
ILQ42_RS18315	3567644..3567840	-	197	Protein_3585	TrmB family transcriptional regulator	-
ILQ42_RS18320	3568127..3568945	-	819	WP_000265265.1	CPBP family intramembrane metalloprotease	-
ILQ42_RS18325	3569097..3569468	-	372	WP_000090264.1	antitermination protein	-
ILQ42_RS18330	3569458..3569829	-	372	WP_001217436.1	RusA family crossover junction endodeoxyribonuclease	-
ILQ42_RS18335	3569842..3570891	-	1050	WP_001265172.1	DUF968 domain-containing protein	-
ILQ42_RS18340	3570893..3571171	-	279	WP_001341388.1	hypothetical protein	-
ILQ42_RS18345	3571339..3571494	-	156	WP_000813263.1	type I toxin-antitoxin system toxin HokD	Toxin
-	3571506..3571564	+	59	-	-	Antitoxin
ILQ42_RS18350	3572099..3572872	+	774	WP_000160654.1	alpha/beta hydrolase	-
ILQ42_RS18355	3573224..3573637	-	414	WP_001151233.1	DUF977 family protein	-
ILQ42_RS18360	3573653..3574423	-	771	WP_000450992.1	DUF1627 domain-containing protein	-
ILQ42_RS18365	3574445..3575191	-	747	WP_000788751.1	ATP-binding protein	-
ILQ42_RS18370	3575198..3576289	-	1092	WP_001205823.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5722.86 Da
• Isoelectric Point: 6.1531

>T176112 WP_000813263.1 NZ_CP062746:c3571494-3571339 [Escherichia coli O157:H7]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRVRTGQTEVAVFTAYEPEE

Nucleotide

• Length: 156 bp

>T176112 NZ_CP082357:2602223-2602330 [Escherichia coli]
ATGACGCTCACGCAGTTTGCCATGACTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT176112 NZ_CP062746:3571506-3571564 [Escherichia coli O157:H7]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTAGATTTGCCGTTCATAGATTGAGCCTCA

Structures

Antitoxin