Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 708408..709207 Replicon chromosome
Accession NZ_CP062746
Organism Escherichia coli O157:H7 strain Z1504

Toxin (Protein)


Gene name yhaV Uniprot ID V0SSH7
Locus tag ILQ42_RS03460 Protein ID WP_000347273.1
Coordinates 708408..708872 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID C3ST72
Locus tag ILQ42_RS03465 Protein ID WP_001302819.1
Coordinates 708872..709207 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ILQ42_RS03430 703409..703843 - 435 WP_000948824.1 PTS sugar transporter subunit IIA -
ILQ42_RS03435 703861..704739 - 879 WP_001295548.1 PTS system mannose/fructose/sorbose family transporter subunit IID -
ILQ42_RS03440 704729..705508 - 780 WP_000406214.1 PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC -
ILQ42_RS03445 705519..705992 - 474 WP_001301475.1 PTS N-acetylgalactosamine transporter subunit IIB -
ILQ42_RS03450 706015..707295 - 1281 WP_000681927.1 tagatose-bisphosphate aldolase subunit KbaZ -
ILQ42_RS03455 707544..708353 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
ILQ42_RS03460 708408..708872 - 465 WP_000347273.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
ILQ42_RS03465 708872..709207 - 336 WP_001302819.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
ILQ42_RS03470 709356..710927 - 1572 WP_001273776.1 galactarate dehydratase -
ILQ42_RS03475 711302..712636 + 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
ILQ42_RS03480 712652..713422 + 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17836.25 Da        Isoelectric Point: 9.6924

>T176093 WP_000347273.1 NZ_CP062746:c708872-708408 [Escherichia coli O157:H7]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T176093 NZ_CP082355:2669077-2669184 [Escherichia coli]
ATGACGCTCACGCAGTTTGCCATGACTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12388.97 Da        Isoelectric Point: 4.8616

>AT176093 WP_001302819.1 NZ_CP062746:c709207-708872 [Escherichia coli O157:H7]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDTDIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT176093 NZ_CP082355:c2669027-2668964 [Escherichia coli]
TCTGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTATACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SSH7

Antitoxin

Source ID Structure

References