Detailed information of TA system TA176079

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 4471106..4471800
• Replicon: chromosome
• Accession: NZ_CP062744
• Organism: Escherichia coli O157:H7 strain Z1615

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: C3TNX7
• Locus tag: ILQ43_RS22570
• Protein ID: WP_001263495.1
• Coordinates: 4471106..4471504 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: ILQ43_RS22575
• Protein ID: WP_000554757.1
• Coordinates: 4471507..4471800 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
-	4466693..4466773	-	81	NuclAT_11	-	-
-	4466693..4466773	-	81	NuclAT_11	-	-
-	4466693..4466773	-	81	NuclAT_11	-	-
-	4466693..4466773	-	81	NuclAT_11	-	-
ILQ43_RS22545	4467369..4467827	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
ILQ43_RS22550	4468088..4469545	+	1458	WP_001293009.1	cytosol nonspecific dipeptidase	-
ILQ43_RS22555	4469602..4470123	-	522	Protein_4415	peptide chain release factor H	-
ILQ43_RS22560	4470122..4470325	-	204	Protein_4416	RNA ligase RtcB family protein	-
ILQ43_RS22565	4470644..4471096	-	453	WP_001059871.1	GNAT family N-acetyltransferase	-
ILQ43_RS22570	4471106..4471504	-	399	WP_001263495.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
ILQ43_RS22575	4471507..4471800	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
ILQ43_RS22580	4471852..4472907	-	1056	WP_001226186.1	DNA polymerase IV	-
ILQ43_RS22585	4472978..4473763	-	786	WP_000207556.1	putative lateral flagellar export/assembly protein LafU	-
ILQ43_RS22590	4473735..4475447	+	1713	Protein_4422	flagellar biosynthesis protein FlhA	-
ILQ43_RS22595	4475592..4476089	-	498	Protein_4423	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15473.86 Da
• Isoelectric Point: 8.0949

>T176079 WP_001263495.1 NZ_CP062744:c4471504-4471106 [Escherichia coli O157:H7]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDESHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T176079 NZ_CP082351:2352074-2352532 [Providencia rettgeri]
ATGATTTTGTATCGATTGGTTAAATCCAGTTTTATTCACGATGCATGGTCTGGTCAAGGAGCCATGCTCTACGGTGGGCG
TTGGAATCATAAAGGAACTCCCGCTGTTTATACTTCAACCAGTATTTCATTAGCCACCCTTGAAATACTCGTTCACATCA
ATCAAGACTTACTATTAAACCAGTTCAGTTTGCTATCCATCGATATCAATGACAGGTACGTGATGAAACTGTCGTTAGAC
CACCTTCCAACAGATTGGCAGCAAGACCCAGCGCCAACTTCTACCATGGATATTGGTACTACATGGCTAAATAACCAAGA
TAGCTTAGTATTATTAATTCCCTCTTGTATTATTCCCTACGAATACAATGCAATTATTAACCCGTTGCACCCGCAATTTC
AAAAAGCCATACATACTGTTAAACCGCTGGATTTTAGTGTTGACTCACGCTTAGCATAG

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT176079 WP_000554757.1 NZ_CP062744:c4471800-4471507 [Escherichia coli O157:H7]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT176079 NZ_CP082351:2351616-2352077 [Providencia rettgeri]
ATGAGAAACTATATCCCCACAACCCCACTTCAACCGACTGCTTCATCTTTTAAACCACTATGGCGCCATGTAGGGTTACC
TGCCGAACGTGGTATTGAATTGACCCACTTCTTACGCCAAGGGCTGTCTGTCTCTGTTTTAGACAATATCCAAGAATGGT
CAGGGATGAGTAAAACTGAATTATTACGGATATCCGGCATTAATGAACGCAATATTGCTCGTCGCAAAAATGCAGGCCAA
CCCCTCAATGCTGATGAAAGCGAACGAATTGCTCGTCTAGTACGCGTATTCGATGCGGCAGTGCGTTTATTTAATGGAGA
TAAACATGCAGCAGGTGATTGGCTTAATCACCCCGTTAAAGGGTTAGGCCATTTACGCCCTATTGAGCTTATTGCGACAG
AAAGTGGAGCCATTGAGGTAATTGATTTGATTGGCCGCATTGAGCATGGTGTGTTTTCATGA

Structures

Antitoxin

• AlphaFold DB: S1FJN6