Detailed information of TA system TA176074

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 3575151..3575376
• Replicon: chromosome
• Accession: NZ_CP062744
• Organism: Escherichia coli O157:H7 strain Z1615

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: -
• Locus tag: ILQ43_RS18375
• Protein ID: WP_000813263.1
• Coordinates: 3575151..3575306 (-)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	3575318..3575376 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ILQ43_RS18340	3570605..3571318	-	714	WP_000261909.1	helix-turn-helix domain-containing protein	-
ILQ43_RS18345	3571456..3571652	-	197	Protein_3590	TrmB family transcriptional regulator	-
ILQ43_RS18350	3571939..3572757	-	819	WP_000265265.1	CPBP family intramembrane metalloprotease	-
ILQ43_RS18355	3572909..3573280	-	372	WP_000090264.1	antitermination protein	-
ILQ43_RS18360	3573270..3573641	-	372	WP_001217436.1	RusA family crossover junction endodeoxyribonuclease	-
ILQ43_RS18365	3573654..3574703	-	1050	WP_001265172.1	DUF968 domain-containing protein	-
ILQ43_RS18370	3574705..3574983	-	279	WP_001341388.1	hypothetical protein	-
ILQ43_RS18375	3575151..3575306	-	156	WP_000813263.1	type I toxin-antitoxin system toxin HokD	Toxin
-	3575318..3575376	+	59	-	-	Antitoxin
ILQ43_RS18380	3575911..3576684	+	774	WP_000160654.1	alpha/beta hydrolase	-
ILQ43_RS18385	3577036..3577449	-	414	WP_001151233.1	DUF977 family protein	-
ILQ43_RS18390	3577465..3578235	-	771	WP_000450992.1	DUF1627 domain-containing protein	-
ILQ43_RS18395	3578257..3579003	-	747	WP_000788751.1	ATP-binding protein	-
ILQ43_RS18400	3579010..3580101	-	1092	WP_001205823.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5722.86 Da
• Isoelectric Point: 6.1531

>T176074 WP_000813263.1 NZ_CP062744:c3575306-3575151 [Escherichia coli O157:H7]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRVRTGQTEVAVFTAYEPEE

Nucleotide

• Length: 156 bp

>T176074 NZ_CP082349:c4237302-4237129 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Nucleotide

• Length: 59 bp

>AT176074 NZ_CP062744:3575318-3575376 [Escherichia coli O157:H7]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTAGATTTGCCGTTCATAGATTGAGCCTCA

Structures

Antitoxin