Detailed information of TA system TA176041

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 4471168..4471862
• Replicon: chromosome
• Accession: NZ_CP062742
• Organism: Escherichia coli O157:H7 strain Z1626

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: C3TNX7
• Locus tag: ILQ44_RS22530
• Protein ID: WP_001263495.1
• Coordinates: 4471168..4471566 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: ILQ44_RS22535
• Protein ID: WP_000554757.1
• Coordinates: 4471569..4471862 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
-	4466755..4466835	-	81	NuclAT_11	-	-
-	4466755..4466835	-	81	NuclAT_11	-	-
-	4466755..4466835	-	81	NuclAT_11	-	-
-	4466755..4466835	-	81	NuclAT_11	-	-
ILQ44_RS22505	4467431..4467889	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
ILQ44_RS22510	4468150..4469607	+	1458	WP_001293009.1	cytosol nonspecific dipeptidase	-
ILQ44_RS22515	4469664..4470185	-	522	Protein_4407	peptide chain release factor H	-
ILQ44_RS22520	4470184..4470387	-	204	Protein_4408	RNA ligase RtcB family protein	-
ILQ44_RS22525	4470706..4471158	-	453	WP_001059871.1	GNAT family N-acetyltransferase	-
ILQ44_RS22530	4471168..4471566	-	399	WP_001263495.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
ILQ44_RS22535	4471569..4471862	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
ILQ44_RS22540	4471914..4472969	-	1056	WP_001226186.1	DNA polymerase IV	-
ILQ44_RS22545	4473040..4473825	-	786	WP_000207556.1	putative lateral flagellar export/assembly protein LafU	-
ILQ44_RS22550	4473797..4475509	+	1713	Protein_4414	flagellar biosynthesis protein FlhA	-
ILQ44_RS22555	4475654..4476151	-	498	Protein_4415	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15473.86 Da
• Isoelectric Point: 8.0949

>T176041 WP_001263495.1 NZ_CP062742:c4471566-4471168 [Escherichia coli O157:H7]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDESHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T176041 NZ_CP082346:c6423-6070 [Pantoea dispersa]
ATGTGGGAAATCATTTTTACCGACACCTTCCGGGAATGGTTGCGGGCACAGGACAACAAACTGAAAAAACGCATCGCTGC
GGCCCTGTTAAACCTGCAGCATTATGGCCCGTTGCTGCCACGGCCCTATGCCGATACGGTTAACGGATCACGCTTCCACA
ACATGAAAGAACTGAGAATACAGTACGCTGGTCAGCCAATTCGGGCGCTATACGCATTCGACACGCAACGCAAAGCGGTC
ATTTTGTGTGCTGGCAATAAATCCGGAGACAAACAATTTTATGCGCGGATGATATGCCTTGCAGATCAATTGTTCACGGC
TTACCTCAATAACGCAGAGGAAAATCATTCATGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT176041 WP_000554757.1 NZ_CP062742:c4471862-4471569 [Escherichia coli O157:H7]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT176041 NZ_CP082346:c6073-5768 [Pantoea dispersa]
ATGAACGACTTACAAAAATTTATCGAGAGTGAGTTTACTGAAGCTGAGCAAGCTGACATTCAGCTGATGGCAGATGAGCT
GATTCTCGAAACCGGCCTGCAACTGCTGCGGGAAGAGTTAGCGTTATCGCAGAGAACCGTTGCGGAAGCGATGGGGGTTA
AGCAGCCGCGTATCACGCAAATTGAGCAGGGCGGCGCCGATCAGCGTCTGATCACCATTAAGCGCTACATTGAAGCGATG
GGCGGCAAGCTAAGCCTGCTGATTGAAATGCCGGACGGCTCGGGTGGAAGAATTCTGCGCGTTTAA

Structures

Antitoxin

• AlphaFold DB: S1FJN6