Detailed information of TA system TA176032

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 2644825..2645050
• Replicon: chromosome
• Accession: NZ_CP062742
• Organism: Escherichia coli O157:H7 strain Z1626

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: A0A1Q4PF16
• Locus tag: ILQ44_RS13435
• Protein ID: WP_000813258.1
• Coordinates: 2644895..2645050 (+)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	2644825..2644883 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ILQ44_RS13385	2639890..2640132	+	243	WP_000747948.1	hypothetical protein	-
ILQ44_RS13390	2640116..2640541	+	426	WP_000693878.1	Rha family transcriptional regulator	-
ILQ44_RS13395	2640610..2641653	+	1044	WP_001262402.1	hypothetical protein	-
ILQ44_RS13400	2641646..2642107	+	462	WP_000139447.1	replication protein	-
ILQ44_RS13405	2642141..2642857	+	717	WP_000450627.1	DUF1627 domain-containing protein	-
ILQ44_RS13410	2642890..2643171	+	282	WP_000603384.1	DNA-binding protein	-
ILQ44_RS13415	2643168..2643395	+	228	WP_000699809.1	hypothetical protein	-
ILQ44_RS13420	2643388..2643699	+	312	WP_001289673.1	hypothetical protein	-
ILQ44_RS13425	2643827..2644045	+	219	WP_000683609.1	DUF4014 family protein	-
ILQ44_RS13430	2644047..2644604	+	558	WP_000104474.1	DUF551 domain-containing protein	-
-	2644825..2644883	-	59	-	-	Antitoxin
ILQ44_RS13435	2644895..2645050	+	156	WP_000813258.1	type I toxin-antitoxin system Hok family toxin	Toxin
ILQ44_RS13440	2645170..2645514	+	345	WP_000756596.1	YgiW/YdeI family stress tolerance OB fold protein	-
ILQ44_RS13445	2645636..2645908	+	273	WP_000191872.1	hypothetical protein	-
ILQ44_RS13450	2645910..2646959	+	1050	WP_001265229.1	DUF968 domain-containing protein	-
ILQ44_RS13455	2646972..2647277	+	306	WP_001217444.1	RusA family crossover junction endodeoxyribonuclease	-
ILQ44_RS13460	2647340..2647894	+	555	WP_000640035.1	DUF1133 family protein	-
ILQ44_RS13465	2648119..2648316	+	198	WP_000917763.1	hypothetical protein	-
ILQ44_RS13470	2648452..2649165	+	714	WP_000301797.1	helix-turn-helix domain-containing protein	-
ILQ44_RS13485	2649616..2650047	+	432	WP_001302123.1	tellurite resistance protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	nleG7' / nleC	2577838..2729755	151917

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5767.95 Da
• Isoelectric Point: 7.7942

>T176032 WP_000813258.1 NZ_CP062742:2644895-2645050 [Escherichia coli O157:H7]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFVDYESEK

Nucleotide

• Length: 156 bp

>T176032 NZ_CP082341:c3717407-3717108 [Pantoea dispersa]
ATGATTGAGGATGTGTTGGCCAGCCTGGCGGTTCTCAGGGCGTTCGGGCCCACGTTAGGGCGGCCTGATGTTGATACCCT
CGTCGGGTCGCGTTTTTCCAATATGAAAGAACTCCGTGTTCAGAGTAATGGCCGGGCGATTCGTGCGTTTTTTGCCTTCG
ATCCGGTCAGACGGGCCATTGTGCTCTGCGCGGGAAACAAAACCGGAACGCATCAGCGGCGCTTTTATCAGGCGATGATT
AAACTGGCTGACCGCGAGTATCAACAGCATCTGGAGGAGATGAATCATGCCAAAACTTGA

Antitoxin

Nucleotide

• Length: 59 bp

>AT176032 NZ_CP062742:c2644883-2644825 [Escherichia coli O157:H7]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACATGTGTTCAGCATGGATTGAGCCTCA

Structures

Toxin

• AlphaFold DB: A0A1Q4PF16

Antitoxin