Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 4681896..4682590 Replicon chromosome
Accession NZ_CP062739
Organism Escherichia coli O157:H7 strain Z1723

Toxin (Protein)


Gene name yafO Uniprot ID C3TNX7
Locus tag ILQ45_RS23785 Protein ID WP_001263495.1
Coordinates 4681896..4682294 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1FJN6
Locus tag ILQ45_RS23790 Protein ID WP_000554757.1
Coordinates 4682297..4682590 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
- 4677483..4677563 - 81 NuclAT_11 - -
- 4677483..4677563 - 81 NuclAT_11 - -
- 4677483..4677563 - 81 NuclAT_11 - -
- 4677483..4677563 - 81 NuclAT_11 - -
ILQ45_RS23760 4678159..4678617 - 459 WP_001291990.1 xanthine phosphoribosyltransferase -
ILQ45_RS23765 4678878..4680335 + 1458 WP_001293009.1 cytosol nonspecific dipeptidase -
ILQ45_RS23770 4680392..4680913 - 522 Protein_4650 peptide chain release factor H -
ILQ45_RS23775 4680912..4681115 - 204 Protein_4651 RNA ligase RtcB family protein -
ILQ45_RS23780 4681434..4681886 - 453 WP_001059871.1 GNAT family N-acetyltransferase -
ILQ45_RS23785 4681896..4682294 - 399 WP_001263495.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
ILQ45_RS23790 4682297..4682590 - 294 WP_000554757.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
ILQ45_RS23795 4682642..4683697 - 1056 WP_001226186.1 DNA polymerase IV -
ILQ45_RS23800 4683768..4684553 - 786 WP_000207556.1 putative lateral flagellar export/assembly protein LafU -
ILQ45_RS23805 4684525..4686237 + 1713 Protein_4657 flagellar biosynthesis protein FlhA -
ILQ45_RS23810 4686382..4686879 - 498 Protein_4658 REP-associated tyrosine transposase RayT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15473.86 Da        Isoelectric Point: 8.0949

>T176005 WP_001263495.1 NZ_CP062739:c4682294-4681896 [Escherichia coli O157:H7]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDESHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T176005 NZ_CP082327:2563049-2563156 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGGATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.81 Da        Isoelectric Point: 5.9050

>AT176005 WP_000554757.1 NZ_CP062739:c4682590-4682297 [Escherichia coli O157:H7]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT176005 NZ_CP082327:c2563002-2562936 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTTTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
AlphaFold DB S1FJN6

References