Detailed information of TA system TA176000

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 3785938..3786163
• Replicon: chromosome
• Accession: NZ_CP062739
• Organism: Escherichia coli O157:H7 strain Z1723

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: -
• Locus tag: ILQ45_RS19595
• Protein ID: WP_000813263.1
• Coordinates: 3785938..3786093 (-)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	3786105..3786163 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ILQ45_RS19560	3781392..3782105	-	714	WP_000261909.1	helix-turn-helix domain-containing protein	-
ILQ45_RS19565	3782243..3782439	-	197	Protein_3826	TrmB family transcriptional regulator	-
ILQ45_RS19570	3782726..3783544	-	819	WP_000265265.1	CPBP family intramembrane metalloprotease	-
ILQ45_RS19575	3783696..3784067	-	372	WP_000090264.1	antitermination protein	-
ILQ45_RS19580	3784057..3784428	-	372	WP_001217436.1	RusA family crossover junction endodeoxyribonuclease	-
ILQ45_RS19585	3784441..3785490	-	1050	WP_001265172.1	DUF968 domain-containing protein	-
ILQ45_RS19590	3785492..3785770	-	279	WP_001341388.1	hypothetical protein	-
ILQ45_RS19595	3785938..3786093	-	156	WP_000813263.1	type I toxin-antitoxin system toxin HokD	Toxin
-	3786105..3786163	+	59	-	-	Antitoxin
ILQ45_RS19600	3786698..3787471	+	774	WP_000160654.1	alpha/beta hydrolase	-
ILQ45_RS19605	3787823..3788236	-	414	WP_001151233.1	DUF977 family protein	-
ILQ45_RS19610	3788252..3789022	-	771	WP_000450992.1	DUF1627 domain-containing protein	-
ILQ45_RS19615	3789044..3789790	-	747	WP_000788751.1	ATP-binding protein	-
ILQ45_RS19620	3789797..3790888	-	1092	WP_001205823.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5722.86 Da
• Isoelectric Point: 6.1531

>T176000 WP_000813263.1 NZ_CP062739:c3786093-3785938 [Escherichia coli O157:H7]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRVRTGQTEVAVFTAYEPEE

Nucleotide

• Length: 156 bp

>T176000 NZ_CP082327:824380-824787 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCTGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT176000 NZ_CP062739:3786105-3786163 [Escherichia coli O157:H7]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTAGATTTGCCGTTCATAGATTGAGCCTCA

Structures

Antitoxin