Detailed information of TA system TA175995

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 2859110..2859335
• Replicon: chromosome
• Accession: NZ_CP062739
• Organism: Escherichia coli O157:H7 strain Z1723

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: A0A1Q4PF16
• Locus tag: ILQ45_RS14670
• Protein ID: WP_000813258.1
• Coordinates: 2859180..2859335 (+)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	2859110..2859168 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ILQ45_RS14620	2854175..2854417	+	243	WP_000747948.1	hypothetical protein	-
ILQ45_RS14625	2854401..2854826	+	426	WP_000693878.1	Rha family transcriptional regulator	-
ILQ45_RS14630	2854895..2855938	+	1044	WP_001262402.1	hypothetical protein	-
ILQ45_RS14635	2855931..2856392	+	462	WP_000139447.1	replication protein	-
ILQ45_RS14640	2856426..2857142	+	717	WP_000450627.1	DUF1627 domain-containing protein	-
ILQ45_RS14645	2857175..2857456	+	282	WP_000603384.1	DNA-binding protein	-
ILQ45_RS14650	2857453..2857680	+	228	WP_000699809.1	hypothetical protein	-
ILQ45_RS14655	2857673..2857984	+	312	WP_001289673.1	hypothetical protein	-
ILQ45_RS14660	2858112..2858330	+	219	WP_000683609.1	DUF4014 family protein	-
ILQ45_RS14665	2858332..2858889	+	558	WP_000104474.1	DUF551 domain-containing protein	-
-	2859110..2859168	-	59	-	-	Antitoxin
ILQ45_RS14670	2859180..2859335	+	156	WP_000813258.1	type I toxin-antitoxin system Hok family toxin	Toxin
ILQ45_RS14675	2859455..2859799	+	345	WP_000756596.1	YgiW/YdeI family stress tolerance OB fold protein	-
ILQ45_RS14680	2859921..2860193	+	273	WP_000191872.1	hypothetical protein	-
ILQ45_RS14685	2860195..2861244	+	1050	WP_001265229.1	DUF968 domain-containing protein	-
ILQ45_RS14690	2861257..2861562	+	306	WP_001217444.1	RusA family crossover junction endodeoxyribonuclease	-
ILQ45_RS14695	2861625..2862179	+	555	WP_000640035.1	DUF1133 family protein	-
ILQ45_RS14700	2862404..2862601	+	198	WP_000917763.1	hypothetical protein	-
ILQ45_RS14705	2862737..2863450	+	714	WP_000301797.1	helix-turn-helix domain-containing protein	-
ILQ45_RS14720	2863901..2864332	+	432	WP_001302123.1	tellurite resistance protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	paa / nleG7' / nleC	2796310..2950609	154299

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5767.95 Da
• Isoelectric Point: 7.7942

>T175995 WP_000813258.1 NZ_CP062739:2859180-2859335 [Escherichia coli O157:H7]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFVDYESEK

Nucleotide

• Length: 156 bp

>T175995 NZ_CP082324:220921-221313 [Thermus thermophilus]
GTGGTCTCCGAGGCCGTCAAGCGGCGGCCTCACCCCAAGGTGGTGGCCTTCCTGCGCCACCTTAAAGTGGGGGAGGCGTA
CCTCTCCGCCCTCACCTTGGGCGAACTGGTCCAGGGGGTGGCCCGGGCCCCGGAAGCCCGAAGGGCCATCCTCAGCCGAT
GGCTCTCGGGGCTGAAGGCGAGCTTCTCGGGGCGGATCCTTCCCCTAGACGCCGAGGTGATGGAGCTCTGGGGCGAGCTC
ACCGGAAACGCCATGAGGGAGGGCAAGCCCCTCTCTCCCCTAGACGCCATGCTGGCGGCCACGACCCTGCGGCACGGCCT
GATCCTGGCCACCCGGAACACCGAGGACTTCCGCCACGTTCCCGTGCCCCTCGTGAACCCCTGGGAAGGGTAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT175995 NZ_CP062739:c2859168-2859110 [Escherichia coli O157:H7]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACATGTGTTCAGCATGGATTGAGCCTCA

Structures

Toxin

• AlphaFold DB: A0A1Q4PF16

Antitoxin