Detailed information of TA system TA175967

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 4468676..4469370
• Replicon: chromosome
• Accession: NZ_CP062736
• Organism: Escherichia coli O157:H7 strain Z1766

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: C3TNX7
• Locus tag: ILQ46_RS22550
• Protein ID: WP_001263495.1
• Coordinates: 4468676..4469074 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: ILQ46_RS22555
• Protein ID: WP_000554757.1
• Coordinates: 4469077..4469370 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
-	4464263..4464343	-	81	NuclAT_11	-	-
-	4464263..4464343	-	81	NuclAT_11	-	-
-	4464263..4464343	-	81	NuclAT_11	-	-
-	4464263..4464343	-	81	NuclAT_11	-	-
ILQ46_RS22525	4464939..4465397	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
ILQ46_RS22530	4465658..4467115	+	1458	WP_001293009.1	cytosol nonspecific dipeptidase	-
ILQ46_RS22535	4467172..4467693	-	522	Protein_4411	peptide chain release factor H	-
ILQ46_RS22540	4467692..4467895	-	204	Protein_4412	RNA ligase RtcB family protein	-
ILQ46_RS22545	4468214..4468666	-	453	WP_001059871.1	GNAT family N-acetyltransferase	-
ILQ46_RS22550	4468676..4469074	-	399	WP_001263495.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
ILQ46_RS22555	4469077..4469370	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
ILQ46_RS22560	4469422..4470477	-	1056	WP_001226186.1	DNA polymerase IV	-
ILQ46_RS22565	4470548..4471333	-	786	WP_000207556.1	putative lateral flagellar export/assembly protein LafU	-
ILQ46_RS22570	4471305..4473017	+	1713	Protein_4418	flagellar biosynthesis protein FlhA	-
ILQ46_RS22575	4473162..4473659	-	498	Protein_4419	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	4430521..4474608	44087

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15473.86 Da
• Isoelectric Point: 8.0949

>T175967 WP_001263495.1 NZ_CP062736:c4469074-4468676 [Escherichia coli O157:H7]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDESHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T175967 NZ_CP082295:86061-86372 [Pantoea agglomerans]
ATGCATGTTATTTCATGCGTGCCGTTTGACGAGGCGGCCAGAAAACATCCCAATGCAGCGGCAGCACTGGATCTGCTTTA
CCGTCACCTCAGACAGGGCGATTTCAGTGGTCCTGACGATCTCAGGGCGCTTTATGCAAGCCTTGATCGTATGAAGTATC
GCGCTAAATGGTGGGTCATTGACGTCAGTGGCAACCATTTGCGCGTGATATTCTTCGCGGATTTTGAGCGCAGCAAAATC
TTCATCAAACATATCTGCACGCACGCTGAGTACGACAAGCTTGTTAAGAAATATCGGGAGGTAACCGAATGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT175967 WP_000554757.1 NZ_CP062736:c4469370-4469077 [Escherichia coli O157:H7]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT175967 NZ_CP082295:86369-86779 [Pantoea agglomerans]
ATGAATACTGAAGCTATCAGGCTGTCAAACCAGCTGGCAGAAGCGGTGCCGCTGCTGGCCGGCAGCAACCAGCGACATGA
TTATCAAGAAGCACTGGAACTGGTCGAATACCTTATTGAACATGAGCCGGATAATCCGCTGGTAGATTTGCTTTGTGCCC
GCATTGCTCATTATGAAGATAATGATCCTGCGATGGCCGACTTCAATAGCCGCCAGCGTGAAATCAGCACCGGACCTGGC
GTTCTGAGGGTATTGATCGAGCAGCACAACCTCAGCCTGAGTGACTTCGAAAATGAAATTGGCAAAAAGTCTCTGGTCAG
CCGAATCCTCAGCGGCGATCGTCAGTTGACCGTGTCACACATACGGGCGTTGTGCGCGCGATTTGGCCTGTCACCATCGT
TGTTTTTTTGA

Structures

Antitoxin

• AlphaFold DB: S1FJN6