Detailed information of TA system TA175957

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 2645888..2646113
• Replicon: chromosome
• Accession: NZ_CP062736
• Organism: Escherichia coli O157:H7 strain Z1766

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: A0A1Q4PF16
• Locus tag: ILQ46_RS13430
• Protein ID: WP_000813258.1
• Coordinates: 2645958..2646113 (+)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	2645888..2645946 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ILQ46_RS13380	2640953..2641195	+	243	WP_000747948.1	hypothetical protein	-
ILQ46_RS13385	2641179..2641604	+	426	WP_000693878.1	Rha family transcriptional regulator	-
ILQ46_RS13390	2641673..2642716	+	1044	WP_001262402.1	hypothetical protein	-
ILQ46_RS13395	2642709..2643170	+	462	WP_000139447.1	replication protein	-
ILQ46_RS13400	2643204..2643920	+	717	WP_000450627.1	DUF1627 domain-containing protein	-
ILQ46_RS13405	2643953..2644234	+	282	WP_000603384.1	DNA-binding protein	-
ILQ46_RS13410	2644231..2644458	+	228	WP_000699809.1	hypothetical protein	-
ILQ46_RS13415	2644451..2644762	+	312	WP_001289673.1	hypothetical protein	-
ILQ46_RS13420	2644890..2645108	+	219	WP_000683609.1	DUF4014 family protein	-
ILQ46_RS13425	2645110..2645667	+	558	WP_000104474.1	DUF551 domain-containing protein	-
-	2645888..2645946	-	59	-	-	Antitoxin
ILQ46_RS13430	2645958..2646113	+	156	WP_000813258.1	type I toxin-antitoxin system Hok family toxin	Toxin
ILQ46_RS13435	2646233..2646577	+	345	WP_000756596.1	YgiW/YdeI family stress tolerance OB fold protein	-
ILQ46_RS13440	2646699..2646971	+	273	WP_000191872.1	hypothetical protein	-
ILQ46_RS13445	2646973..2648022	+	1050	WP_001265229.1	DUF968 domain-containing protein	-
ILQ46_RS13450	2648035..2648340	+	306	WP_001217444.1	RusA family crossover junction endodeoxyribonuclease	-
ILQ46_RS13455	2648403..2648957	+	555	WP_000640035.1	DUF1133 family protein	-
ILQ46_RS13460	2649182..2649379	+	198	WP_000917763.1	hypothetical protein	-
ILQ46_RS13465	2649515..2650228	+	714	WP_000301797.1	helix-turn-helix domain-containing protein	-
ILQ46_RS13480	2650679..2651110	+	432	WP_001302123.1	tellurite resistance protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	paa / nleG7' / nleC	2583087..2729029	145942

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5767.95 Da
• Isoelectric Point: 7.7942

>T175957 WP_000813258.1 NZ_CP062736:2645958-2646113 [Escherichia coli O157:H7]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFVDYESEK

Nucleotide

• Length: 156 bp

>T175957 NZ_CP082290:c5078299-5077916 [Klebsiella pneumoniae subsp. pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT175957 NZ_CP062736:c2645946-2645888 [Escherichia coli O157:H7]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACATGTGTTCAGCATGGATTGAGCCTCA

Structures

Toxin

• AlphaFold DB: A0A1Q4PF16

Antitoxin