Detailed information of TA system TA175931

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 4468644..4469338
• Replicon: chromosome
• Accession: NZ_CP062733
• Organism: Escherichia coli O157:H7 strain Z1767

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: C3TNX7
• Locus tag: ILQ47_RS22545
• Protein ID: WP_001263495.1
• Coordinates: 4468644..4469042 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: ILQ47_RS22550
• Protein ID: WP_000554757.1
• Coordinates: 4469045..4469338 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
-	4464231..4464311	-	81	NuclAT_11	-	-
-	4464231..4464311	-	81	NuclAT_11	-	-
-	4464231..4464311	-	81	NuclAT_11	-	-
-	4464231..4464311	-	81	NuclAT_11	-	-
ILQ47_RS22520	4464907..4465365	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
ILQ47_RS22525	4465626..4467083	+	1458	WP_001293009.1	cytosol nonspecific dipeptidase	-
ILQ47_RS22530	4467140..4467661	-	522	Protein_4410	peptide chain release factor H	-
ILQ47_RS22535	4467660..4467863	-	204	Protein_4411	RNA ligase RtcB family protein	-
ILQ47_RS22540	4468182..4468634	-	453	WP_001059871.1	GNAT family N-acetyltransferase	-
ILQ47_RS22545	4468644..4469042	-	399	WP_001263495.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
ILQ47_RS22550	4469045..4469338	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
ILQ47_RS22555	4469390..4470445	-	1056	WP_001226186.1	DNA polymerase IV	-
ILQ47_RS22560	4470516..4471301	-	786	WP_000207556.1	putative lateral flagellar export/assembly protein LafU	-
ILQ47_RS22565	4471273..4472985	+	1713	Protein_4417	flagellar biosynthesis protein FlhA	-
ILQ47_RS22570	4473130..4473627	-	498	Protein_4418	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	4430490..4474576	44086

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15473.86 Da
• Isoelectric Point: 8.0949

>T175931 WP_001263495.1 NZ_CP062733:c4469042-4468644 [Escherichia coli O157:H7]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDESHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T175931 NZ_CP082272:1670090-1670380 [Pasteurella multocida]
ATGAGTAAAAAAGTAGAATTGAAGCCATTTGATATTGCTGAACATCTAGATAGTGAAGAAATGATTGCCGCATATTTAAG
TGAAGTTTTAGAAACAGGTGATACTAATGAATTTATTTCCGCGCTAGGTGATGTTGCAAGAGCAAGAGGAATGACGGAGT
TAGCAGCAAAAACAGGGTTGGGAAGAGAAAGTCTTTATAAAACATTATCATACGGTAGTAAGCCACGTTTTGATACTATA
ATGAAAATTACGCAAGCATTAGGTATTAAACTTGTCCCAACGCACATCTAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT175931 WP_000554757.1 NZ_CP062733:c4469338-4469045 [Escherichia coli O157:H7]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT175931 NZ_CP082272:1669779-1670093 [Pasteurella multocida]
ATGTTTGAGCTAATTGAAACAACAGTATTTAATAAATGGCTTAAAGAGCTAAAAGATTTGTCGGCTAAGGCGGCTATTCT
TGCTCGTATTAATAGAGCTAAAAATGGTAATTTTGGCGATCACAAATCAGTTGGTGATGGCTTGTATGAAATGAGAATAA
TGAAAGGAGCTGGTTATAGGGTTTATTACACACAATACAAAGATGTAACCTATTTGTTAATTTGCGGAGGTGATAAATCT
ACACAGAAAGCAGACATCGCTAAAGCAAAAGTTTTATGGGAAGAGATTAAGCAGAAAGAGGGGGTAAATGTATGA

Structures

Antitoxin

• AlphaFold DB: S1FJN6